Ying MicroRNA Protocols

Third Auflage 2018
ISBN: 978-1-4939-7601-0
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1733, 319 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume focuses on the analysis of miRNA and its many components such as targets and expression profiling, regulation of gene expression, miRNA detection in biofluids, and its application in cancer and wound healing. The chapters in this book explore topics that’s cover biogenesis, isolation and profiling of miRNA, exosomal miRNAs, and the study of the functional significance of miRNAs. The chapters also cover screening miRNAs in mR-302/367 induced iPSCs, exosomal miRNAs from iPSC-derived cardiomyocytes proliferation, and the recently discovered sugar-like RNA glycylglycerins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, MicroRNA Protocols, Third Edition is a valuable resource thatprovides researchers with a comprehensive study of miRNAs and its numerous components and uses.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Ying, Shao-Yao

Inhaltsverzeichnis

The MicroRNA.- Target mRNA Driven Biogenesis of Cognate MicroRNAs In Vitro.- Isolation of Viral-Infected Brain Regions for miRNA Profiling from Formalin-Fixed Paraffin-Embedded Tissues by Laser Capture Microdissection .- Isolation and Analysis of Exosomal Microsomal MicroRNAs from Ovarian Follicular Fluid.- Profiling of MicroRNAs in the Biofluids of Livestock Species.- Exosomal MicroRNAs as Potential Biomarkers in Neuropsychiatric Disorders.- Identification and Validation of Potential Differential miRNA Regulation via Alternative Polyadenylation.- How to Explore the Function and Importance of MicroRNAs: MicroRNAs Expression Profile and their Target/Pathway Prediction in Bovine Ovarian Cells.- Gene Silencing In Vitro and In Vivo using Intronic MicroRNAs.- Mining Exosomal MicroRNAs from Human Induced Pluripotent Stem Cells-Derived Cardiomyocytes for Cardiac Regeneration.- Quantitative Analysis of Precursors MicroRNAs and their Respective Mature MicroRNAs in Cancer Exosomes Overtime.- Quantum Language of MicroRNA: Application for New Cancer Therapeutic Targets.- In Vitro Methods for Analyzing miRNA Roles in Cancer Cell Proliferation, Invasion,  and Metastasis.- Isolation and Identification of Gene-Specific MicroRNAs.- Comprehensive Measurement of Gene Silencing Involving Endogenous MicroRNAs in Mammalian Cells.- Screening miRNA for Functional Significance by 3D Cell Culture System.- Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs.- Neonatal Rat Cardiomyocytes Isolation, Culture and Determination of MicroRNAs’ Effects in Proliferation.- Gene Manipulation with Micro RNAs at Single Human Cancer Cell.- Laser Capture Microdissection of Epithelium from a Wound Healing Model for MicroRNA Analysis.- Transgene-Like Animal Models Using Intronic MicroRNAs.- Application of TALE-Based Approach for Dissecting Functional MicroRNA-302/367 in Cellular Reprogramming.- Mechanism and Method for Generating Tumor-Free iPS Cells Using Intronic MicroRNA miR302 Induction.- The miR-302-Mediated Induction of Pluripotent Stem Cells (iPSC): Multiple Synergistic Reprogramming Mechanisms.- Identification and Isolation of Novel Sugar-Like RNA Protecting Materials Glycylglycerins from Pluripotent Stem Cells.