Wu / Allis Chromatin and Chromatin Remodeling Enzymes, Part B

1. Auflage 2004
ISBN: 978-0-08-049714-3
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 458 Seiten, Web PDF

DNA in the nucleus of plant and animal cells is stored in the form of chromatin. Chromatin and the Chromatin remodelling enzymes play an important role in gene transcription.

Autoren/Hrsg.

Wu, Carl

Allis, C. David

Inhaltsverzeichnis

1;Cover;1
2;Table of Contents;6
3;Contributors to Volume 376;10
4;Preface;16
5;Volumes in Series;18
6;Section I: Biophysics, Structural Biology, and Enzymology of Chromatin Proteins;40
6.1;Chapter 1. Fluorescence Anisotropy Assays for Analysis of ISWI-DNA and ISWI-Nucleosome Interactions;42
6.2;Chapter 2. Biophysical Analysis of Specific Genomic Loci Assembled as Chromatin In Vivo;56
6.3;Chapter 3. Visualization and 3D Structure Determination of Defined Sequence Chromatin and Chromatin Remodeling Complexes;68
6.4;Chapter 4. Electron Microscopic Analysis of the RSC Chromatin Remodeling Complex;87
6.5;Chapter 5. Use of Optical Trapping Techniques to Study Single-Nucleosome Dynamics;101
6.6;Chapter 6. Single-Molecule Analysis of Chromatin;112
6.7;Chapter 7. Biochemical and Structural Characterization of Recombinant Histone Acetyltransferase Proteins;145
6.8;Chapter 8. Use of Nuclear Magnetic Resonance Spectroscopy to Study Structure-Function of Bromodomains;158
6.9;Chapter 9. Assays for the Determination of Structure and Dynamics of the Interaction of the Chromodomain with Histone Peptides;170
6.10;Chapter 10. Expression, Purification, and Biophysical Studies of Chromodomain Proteins;187
6.11;Chapter 11. Quantitative Assays for Characterization of the Sir2 Family of NAD+-Dependent Deacetylases;210
6.12;Chapter 12. Selective HAT Inhibitors as Mechanistic Tools for Protein Acetylation;227
6.13;Chapter 13. Histone Deacetylase Inhibitors: Assays to Assess Effectiveness In Vitro and In Vivo;238
7;Section II: Immunochemical Assays of Chromatin Functions;246
7.1;Chapter 14. Immunochemical Analysis of Chromatin;248
7.2;Chapter 15. Generation and Characterization of Antibodies Directed Against Di-Modified Histones, and Comments on Antibody and Epitope Recognition;260
7.3;Chapter 16. Generation and Characterization of Methyl-Lysine Histone Antibodies;273
7.4;Chapter 17. Tips in Analyzing Antibodies Directed Against Specific Histone Tail Modifications;294
7.5;Chapter 18. Histone Methylation: Recognizing the Methyl Mark;308
7.6;Chapter 19. Analysis of Genome-Wide Histone Acetylation State and Enzyme Binding Using DNA Microarrays;328
7.7;Chapter 20. Use of Chromatin Immunoprecipitation Assays in Genome-Wide Location Analysis of Mammalian Transcription Factors;343
7.8;Chapter 21. High-Throughput Screening of Chromatin Immunoprecipitates Using CpG-Island Microarrays;354
7.9;Chapter 22. Chromatin Immunoprecipitation in the Analysis of Large Chromatin Domains Across Murine Antigen Receptor Loci;373
7.10;Chapter 23. The Use of Chromatin Immunoprecipitation Assays in Genome-Wide Analyses of Histone Modifications;388
7.11;Chapter 24. Sequential Chromatin Immunoprecipitation from Animal Tissues;400
7.12;Chapter 25. Immunofluorescent Staining of Polytene Chromosomes: Exploiting Genetic Tools;411
7.13;Chapter 26. Indirect Immunofluorescent Labeling of Drosophila Polytene Chromosomes: Visualizing Protein Interactions with Chromatin In Vivo;432
7.14;Chapter 27. X-Chromosome Inactivation in Mouse Embryonic Stem Cells: Analysis of Histone Modifications and Transcriptional Activity Using Immunofluorescence and FISH;444
7.15;Chapter 28. X Inactivation in Mouse ES Cells: Histone Modifications and FISH;458
8;Author Index;468
9;Subject Index;488