Wu / Allis Chromatin and Chromatin Remodeling Enzymes, Part A

1. Auflage 2004
ISBN: 978-0-08-049713-6
Verlag: Elsevier Science & Techn.
Format: EPUB
Kopierschutz: 6 - ePub Watermark

E-Book, Englisch, Band Volume 375, 540 Seiten

Reihe: Methods in Enzymology

DNA in the nucleus of plant and animal cells is stored in the form of chromatin. Chromatin and the Chromatin remodellng enzymes play an important role in gene transcription.*Histone Bioinformatics*Biochemistry of histones, nucleosomes and chromatin*Molecular cytology of chromatin functions

Autoren/Hrsg.

Wu, Carl

Allis, C. David

Inhaltsverzeichnis

1;Cover;1
2;Chromatin and Chromatin Remodeling Enzymes;4
3;Copyright Page;5
4;Table of Contents;6
5;Contributors to Volume 375;10
6;Preface;16
7;Volumes in Series;18
8;Section I: Histone Bioinformatics;40
8.1;Chapter 1. Mining Core Histone Sequences from Public Protein Databases;42
9;Section II: Biochemistry of Histones, Nucleosomes, and Chromatin;60
9.1;Chapter 2. Reconstitution of Nucleosome Core Particles from Recombinant Histones and DNA;62
9.2;Chapter 3. Preparation and Crystallization of Nucleosome Core Particle;83
9.3;Chapter 4. Creating Designer Histones by Native Chemical Ligation;101
9.4;Chapter 5. Two-Dimensional Gel Analysis of Histones and Other H2AX-Related Methods;115
9.5;Chapter 6. Assembly of Yeast Chromatin Using ISWI Complexes;127
9.6;Chapter 7. Reconstitution of Yeast Chromatin Using yNap1p;142
9.7;Chapter 8. DNA Synthesis-Dependent and -Independent Chromatin Assembly Pathways in Xenopus Egg Extracts;156
9.8;Chapter 9. Preparation of Defined Mononucleosomes, Dinucleosomes, and Nucleosome Arrays In Vitro and Analysis of Transcription Factor Binding;170
9.9;Chapter 10. Purification of Native, Defined Chromatin Segments;197
9.10;Chapter 11. Purification of Defined Chromosomal Domains;209
9.11;Chapter 12. Probing Core Histone Tail–DNA Interactions in a Model Dinucleosome System;218
9.12;Chapter 13. Site-Directed Histone–DNA Contact Mapping for Analysis of Nucleosome Dynamics;232
9.13;Chapter 14. Site-Specific Attachment of Reporter Compounds to Recombinant Histones;250
9.14;Chapter 15. Histone MacroH2A Purification and Nucleosome Reconstitution;267
9.15;Chapter 16. Analysis of Histone Variant H2A.Z Localization and Expression during Early Development;278
9.16;Chapter 17. Histone Variant CENP-A Purification, Nucleosome Reconstitution;292
9.17;Chapter 18. Immunological Analysis and Purification of Centromere Complex;309
9.18;Chapter 19. Purification and Analyses of Histone H1 Variants and H1 Posttranslational Modifications;317
9.19;Chapter 20. HMGA Proteins: Isolation, Biochemical Modifications, and Nucleosome Interactions;336
9.20;Chapter 21. Preparation and Functional Analysis of HMGN Proteins;362
10;Section III: Molecular Cytology of Chromatin Functions;382
10.1;Chapter 22. Methods for Visualizing Chromatin Dynamics in Living Yeast;384
10.2;Chapter 23. Direct Visualization of Transcription Factor-Induced Chromatin Remodeling and Cofactor Recruitment In Vivo;405
10.3;Chapter 24. Measuring Histone and Polymerase Dynamics in Living Cells;420
10.4;Chapter 25. Measurement of Dynamic Protein Binding to Chromatin In Vivo, Using Photobleaching Microscopy;432
10.5;Chapter 26. Quantification of Protein–Protein and Protein–DNA Interactions In Vivo, Using Fluorescence Recovery after Photobleaching;454
10.6;Chapter 27. Fluorescence Recovery after Photobleaching (FRAP) Methods for Visualizing Protein Dynamics in Living Mammalian Cell Nuclei;482
10.7;Chapter 28. Correlative Light and Electron Spectroscopic Imaging of Chromatin In Situ;495
10.8;Chapter 29. RNA Fluorescence In Situ Hybridization Tagging and Recovery of Associated Proteins to Analyze In Vivo Chromatin Interactions;518
10.9;Chapter 30. 3C Technology: Analyzing the Spatial Organization of Genomic Loci In Vivo;532
11;Author Index;548
12;Subject Index;566