Intermediate Filament Associated Proteins

Erscheinungsjahr 2016
ISBN: 978-0-12-803469-9
Verlag: ACADEMIC PR INC

Buch, Englisch, Band 569, 602 Seiten, Format (B × H): 155 mm x 231 mm, Gewicht: 1179 g

Reihe: Methods in Enzymology

Intermediate Filament Associated Proteins, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in intermediate filament associated proteins and contains sections on such topics as lamin-associated proteins, intermediate filament-associated proteins and plakin, and other cytoskeletal cross-linkers.

Autoren/Hrsg.

Wilson, Katherine

Sonnenberg, Arnoud

Weitere Mitwirkende

Wilson, Katherine L.

Sonnenberg, Arnoud

Fachgebiete

• Naturwissenschaften Biowissenschaften Enzymologie
• Naturwissenschaften Biowissenschaften Proteinforschung

Inhaltsverzeichnis

Part I: Identification and Biochemical Analysis of Lamin-Associated Proteins

1. BioID Identification of Lamin-Associated Proteins
Aaron A. Mehus, Ruthellen H. Anderson and Kyle J. Roux
2. Proximity-Dependent Biotin Identification (BioID) in Dictyostelium Amoebae
Petros Batsios, Irene Meyer and Ralph Gräf
3. Purification and Structural Analysis of LEM-Domain Proteins
Isaline Herrada, Benjamin Bourgeois, Camille Samson, Brigitte Buendia, Howard J Worman and Sophie Zinn-Justin
4. Purification and Structural Analysis of SUN and KASH Domain Proteins
F. Esra Demircioglu, Victor E. Cruz and Thomas U. Schwartz
5. Purification of Lamins and Soluble Fragments of NETs
Alexandr A. Makarov, Andrea Rizzotto, Peter Meinke and Eric C. Schirmer
6. Simple Separation of Functionally-Distinct Populations of Lamin-Binding Proteins
Jason M. Berk and Katherine L. Wilson
Part II: General Methods for Analysis of Intermediate Filament-Associated Proteins

7. Development of a Novel Green Fluorescent Protein-Based Binding Assay to Study the Association of Plakins with Intermediate Filament Proteins
Bertrand Favre, Nadja Begré, Jamal-Eddine Bouameur, and Luca Borradori
8. Functional Analysis of Keratin-Associated Proteins in Intestinal Epithelia: Heat- Shock Protein Chaperoning and Kinase Rescue
Anastasia Mashukova, Radia Forteza and Pedro J. Salas
9. Purification of Protein Chaperones and their Functional Assays with Intermediate Filaments
Ming Der Perng, Yu-Shan Huang and Roy A. Quinlan
10. Purification and Structural Analysis of Plectin and BPAG1e
José A Manso, Inés García Rubio, María Gómez-Hernández, Esther Ortega, Rubén M  Buey, Ana M Carballido, Arturo Carabias, Noelia Alonso-García and José M de Pereda

11. Purification and Structural Analysis of Desmoplakin
Hee-Jung Choi and William I. Weis
12. Degradation of the Intermediate Filament Family by Gigaxonin
Pascale Bomont
Part III: Functional and Genetic Analysis of the Plakin and Other Cytoskeletal Cross-Linkers

13. Functional and Genetic Analysis of Plec

Über Autor(innen)

Wilson, Katherine L.
Department of Cell Biology, The Johns Hopkins University School of Medicine, USA

Sonnenberg, Arnoud
Department of Cell Biology, The Netherlands Cancer Institute, The Netherlands