A guide to methods and experimental analysis
Buch, Englisch, 356 Seiten, Format (B × H): 160 mm x 241 mm, Gewicht: 1540 g
ISBN: 978-0-412-43400-6
Verlag: Springer Netherlands
Building from general principles, the authors clearly explain the fundamental role of epithelia in plasma electrolyte and water balance. Emphasis is placed on experimental approaches and methodology. A comprehensive glossary of terms is included.
Zielgruppe
Research
Autoren/Hrsg.
Fachgebiete
- Naturwissenschaften Biowissenschaften Tierkunde / Zoologie Tierethologie
- Naturwissenschaften Biowissenschaften Proteinforschung
- Naturwissenschaften Biowissenschaften Zellbiologie
- Naturwissenschaften Biowissenschaften Molekularbiologie
- Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
Weitere Infos & Material
1 Epithelial structure and function.- 2 Epithelial transport proteins.- 3 Epithelial polarity.- 4 Characterization of epithelial ion transport.- 5 Epithelial electrophysiology.- 6 Impedance analysis of epithelia.- 7 Measurements and interpretation of cytoplasmic ion activities.- 8 Measurements of epithelial cell volume.- 9 Methods and experimental analysis of isolated epithelial cell membranes.- 10 Methods and experimental analysis of single ion channels.- 11 Epithelial cell culture.- 12 Signaling pathways regulating ion transport in polarized cells.- 13 The cytoskeleton and epithelial function.- 14 Future perspectives: molecular biology and pathophysiology.- Appendix A: Instrumentation.- Appendix B: Solving simple equivalent circuits.- Appendix C: A spread-sheet for marker distribution data.
