PCR Detection of Microbial Pathogens

2. Auflage 2012
ISBN: 978-1-60327-352-7
Verlag: Humana Press

Buch, Englisch, Band 943, 317 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 7684 g

Reihe: Methods in Molecular Biology

PCR methods for the detection of microbial pathogens have made relatively little impact in diagnostic microbiology laboratories due to the common decision to use expensive commercially produced tests rather than the cheaper alternative of developing one’s own tests or introducing tests developed by other workers. PCR Detection of Microbial Pathogens, Second Edition presents alternatives to commercially produced PCR methods to detect microbial pathogens. Although most of the chapters in this book are devoted to the detection of specific pathogens, the first  chapters in this book should appeal to anyone working in this field regardless of their particular interests. Although PCR tests can often be made to work with relatively little effort, it is often unclear how efficient the PCR test is, how inhibitory the specimen containing the pathogen of interest is and how the test can be quality controlled. All of which are of great importance in developing tests for diagnostic use. These topics are covered  in great depth at the beginning of the book. The main part of the book is devoted to describing methods for the detection of a wide range of pathogens and from widely different specimens and situations. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
 
Authoritative and accessible, PCR Detection of Microbial Pathogens, Second Edition serves microbiologists regardless of their particular interest because, when used together with the general principles, the sheer variety of procedures provided here enables the reader to design and introduce diagnostic tests in the laboratory with confidence.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Wilks, Mark

Fachgebiete

• Naturwissenschaften Biowissenschaften Mikrobiologie
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Bakteriologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Molekulare Medizin, Zellbiologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Medizinische Mikrobiologie & Virologie
• Naturwissenschaften Biowissenschaften Zellbiologie

Inhaltsverzeichnis

1. Real-time Quantitative PCR, Pathogen Detection and MIQE

            Jamie Murphy, Tania Nolan  and Stephen A. Bustin

2. Overcoming Inhibition in Real-time Diagnostic PCR

            Johannes Hedman and Peter Rådström

3. Quality in the Molecular Microbiology Laboratory  

            Paul S. Wallace and William G. MacKay

4. Pre-Analytical Sample Treatment and DNA Extraction Protocols for the Detection of Bacterial Pathogens from Whole Blood

            Wendy L. J. Hansen, Cathrien A. Bruggeman, and Petra F. G. Wolffs

5. Detection of Bacterial Contamination in Platelet Concentrates using Flow Cytometry and Real-time PCR Methods

            Tanja Vollmer, Knut Kleesiek, and Jens Dreier

6. Multiplex Real-time PCR Assay for the Detection of Meticillin-Resistant Staphylococcus aureus and Panton-Valentine Leukocidin from Clinical Samples

            Lynne Renwick, Anne Holmes, and Kate Templeton

7. PCR Detection of Haemophilus influenzae from Respiratory Specimens

            Guma M. K. Abdeldaim and Björn Herrmann

8.  Rapid Detection of Atypical Respiratory Bacterial Pathogens by Real-time PCR

            Clare L. Ling and Timothy D. McHugh

9. Use of Multiple Real-time PCR Assays for the Diagnosis of Bodetella spp in Clinical Specimens

            Kathleen M. Tatti and M. Lucia Tondella

10. Detection of Mycoplasma pneumoniae by Real-time PCR

            Jonas M. Winchell and Stephanie L. Mitchell

11. Real-Time PCR Assay for the Diagnosis of Pneumocystis jirovecii Pneumonia

            Judith Fillaux and Antoine Berry

12. Rapid Identification of Mycobacteria and Rapid Detection of Drug Resistance in Mycobacterium tuberculosis in Cultured Isolates and in Respiratory Specimens

            Wing-Cheong Yam and Kit-Hang Gilman Siu

13. Direct Detection of Mycobaterium ulcerans in Clinical Specimens and Environmental Samples

            Caroline J. Lavender and Janet A.M. Fyfe

14. Detection of Bartonella spp. DNA in Clinical Specimens using an Internally Controlled Real-time PCR Assay

            Anneke M. C. Bergmans and  John W.A. Rossen

15. Direct Identification of Multiple Genital Pathogens in Urine using Multiplex PCR-based Reverse Line Blot Assays

            Michelle L. McKechnie, Fanrong Kong, and Gwendolyn L. Gilbert

16. Diagnosis of Clostridium difficile Infection using Real-time PCR

            Renate Johanna van den Berg,  Dennis Bakker, and Ed J. Kuijper

17. Detection of Pathogenic Leptospira spp. through Real-time PCR (qPCR) Targeting the LipL32 Gene

            Robyn Anne Stoddard

18. Sensitive and Rapid Detection of Campylobacter jejuni and Campylobacter coli using Loop-mediated Isothermal Amplification

            Wataru Yamazaki

19. PCR Detection of Helicobacter pylori in Clinical Samples

            Emiko Rimbara, Masanori Sasatsu, and David Y. Graham

20. Rapid Detection of the Escherichia coli Genospecies in Water by Conventional and Real-time PCR

            Andrée F. Maheux, Luc Bissonnette, and Michel G. Bergeron

21. Multiplex Real Time PCR (MRT-PCR) for Diarrheagenic Escherichia coli

            Francesca Barletta, Theresa J. Ochoa,  and Thomas G. Cleary