Wilkins / Appel / Williams Proteome Research

Concepts, Technology and Application
2. Auflage 2007
ISBN: 978-3-540-72910-5
Verlag: Springer Berlin Heidelberg
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 239 Seiten

Reihe: Principles and Practice

Proteomics is a multifaceted, interdisciplinary field which studies the complexity and dynamics of proteins in biological systems. It combines powerful separation and analytical technology with advanced informatics to understand the function of proteins in the cell and in the body. This book provides a clear conceptual description of each facet of proteomics, describes recent advances in technology and thinking in each area, and provides details of how these have been applied to a variety of biological problems. It is written by expert practitioners in the field, from industry, research institutions, and the clinic. It provides junior and experienced researchers with an invaluable proteomic reference, and gives fascinating glimpses of the future of this dynamic field.

Autoren/Hrsg.

Wilkins, M. R.

Appel, R. D.

Williams, K. L.

Hochstrasser, D. F.

Inhaltsverzeichnis

1;Preface to Second Edition;6
2;Foreword to Second Edition;7
3;Contributors;10
4;Contents;13
5;1 Ten Years of the Proteome;19
5.1;Abstract;19
5.2;1.1 Introduction to the Proteome;19
5.3;1.2 Proteomics Is Technology-Driven;21
5.4;1.3 What Has Proteomics Delivered?;26
5.5;1.4 What Still Eludes Us?;27
5.6;1.5 This Book and Some Conclusions;29
5.7;References;29
6;2 Sample Preparation and Prefractionation Techniques for Electrophoresis- Based Proteomics;32
6.1;Abstract;32
6.2;2.1 Introduction;32
6.3;2.2 Conventional Sample Preparation;33
6.4;2.3 Artefacts;35
6.5;2.4 Multiplexed Approaches to Proteomics;39
6.6;2.5 Prefractionation Tools;41
6.7;2.6 Other Methods for Prefractionation of Samples;47
6.8;2.7 Conclusions;52
6.9;References;53
7;3 Protein Identification in Proteomics;58
7.1;Abstract;58
7.2;3.1 Introduction;58
7.3;3.2 Attributes of Proteins Useful for Their Identification;59
7.4;3.3 Protein Identification by Mass Spectrometry;62
7.5;3.4 List of Tools and URLs;82
7.6;3.5 Concluding Remarks;82
7.7;References;83
8;4 Quantitation in Proteomics;85
8.1;Abstract;85
8.2;4.1 Introduction;85
8.3;4.2 Non-mass-spectrometric Approaches to Quantitation;86
8.4;4.3 Relative Quantitation by Mass Spectrometry;90
8.5;4.4 Analysis of Known Post-translational Modifications;99
8.6;4.5 Conclusions;103
8.7;References;104
9;5 One Gene, Many Proteins;110
9.1;Abstract;110
9.2;5.1 Introduction;110
9.3;5.2 An Overview of Modifications: What Are They and Where Do They Occur?;114
9.4;5.3 How Do We Find Post-translational Modifications?;115
9.5;5.4 Analysis of Specific Modifications;120
9.6;5.5 The Function of Protein Post-translational Modifications: More Than Meets the Eye?;124
9.7;5.6 Some Interesting Modification Stories;126
9.8;5.7 Future Directions;131
9.9;References;131
10;6 Proteome Imaging;137
10.1;Abstract;137
10.2;6.1 Introduction;137
10.3;6.2 Image Analysis of Two-Dimensional Electrophoresis Gels;138
10.4;6.3 Liquid Chromatography–Mass Spectrometry;144
10.5;6.4 The Molecular Scanner;148
10.6;6.5 Imaging Mass Spectrometry;152
10.7;6.6 Conclusion;155
10.8;References;156
11;7 Data Integration in Proteomics;159
11.1;Abstract;159
11.2;7.1 Introduction;159
11.3;7.2 Integration As Gathering and Cross-Linking Information;162
11.4;7.3 Integration As Blending of Information;173
11.5;7.4 Concluding Remarks;178
11.6;References;178
12;8 Protein–Protein Interactions;183
12.1;Abstract;183
12.2;8.1 Introduction;183
12.3;8.2 Protein–Protein Interactions in Human Diseases: Altered Protein Connectivity Leads to Disorder;184
12.4;8.3 Charting Protein–Protein Interactions;186
12.5;8.4 Biological and Biomedical Applications;195
12.6;8.5 Future Directions;200
12.7;References;201
13;9 Biomedical Applications of Proteomics;207
13.1;Abstract;207
13.2;9.1 Introduction;207
13.3;9.2 The Application of Proteomics to Medicine;208
13.4;9.3 Disease Diagnosis from Body Fluids;210
13.5;9.4 Vascular Diseases;211
13.6;9.5 Neurodegenerative Disorders;216
13.7;9.6 Proteomics and Cancer;220
13.8;9.7 Toxicopharmacology: the Example of Type 2 Diabetes;225
13.9;9.8 Current Limitations and Future Directions of Proteomics for Medicine;229
13.10;9.9 Present and Future Directions;231
13.11;References;231
14;10 Proteomics: Where to Next?;236
14.1;Abstract;236
14.2;10.1 Introduction;236
14.3;10.2 The Relevance of -omics to Biology;237
14.4;10.3 Technological Developments in Proteomics;238
14.5;10.4 The Next Steps for Proteomics: Diagnostics and Drugs;240
14.6;10.5 Conclusions;242
14.7;References;242
15;Index;244