E-Book, Englisch, 460 Seiten, eBook
Reihe: Springer Lab Manuals
Wegner Diagnostic Cytogenetics
Erscheinungsjahr 2013
ISBN: 978-3-642-59918-7
Verlag: Springer
Format: PDF
Kopierschutz: 1 - PDF Watermark
E-Book, Englisch, 460 Seiten, eBook
Reihe: Springer Lab Manuals
ISBN: 978-3-642-59918-7
Verlag: Springer
Format: PDF
Kopierschutz: 1 - PDF Watermark
In addition to the traditional cytogenetics still used as the basic methodology for everyday clinical diagnosis, new molecular cytogenetic techniques provide a useful basis for routine diagnosis. Flourescence in situ hybridization (FISH) has become a standard technique, and comparative genomic hybridization (CGH), spectral karyotyping (SKY), and multi-color FISH have shown their potential for diagnostic purposes. Following a section on tissue culture, chromosome staining and basic information about karyotyping, nomenclature and quality standards, protocols of relevance for comprehensive cytogenetic diagnostics are presented.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Weitere Infos & Material
I Classical Cytogenetics.- 1 Tissue Culture.- 2 Chromosome Staining.- 3 Karyotyping and Data Interpretation.- 4 Documentation.- II Postnatal Diagnosis.- 5 Peripheral Blood.- 6 Establishment of Permanent Growing Lymphoblastoid Cell Lines.- 7 Solid Tissues.- 8 Cells from Urine Sample.- 9 Classical and Molecular Cytogenetics of Tumor Cells.- 10 Cytogenetics of Meiotic Cells.- III Prenatal Diagnosis.- 11 Prenatal Diagnosis — An Introduction.- 12 Amniotic Fluid Cell Analysis.- 13 Chorionic Villi Sampling.- IV Special Applications.- 14 Diagnosis of Chromosomal Instability Syndromes.- 15 Flow Cytometric Testing for Syndromes with Chromosomal Instability, Aplastic Anemia and Related Hematological Disorders.- 16 Cell Fusion.- 17 Origin of Trisomies.- V Molecular Cytogenetics.- 18 Fluorescence in Situ Hybridization.- 19 Fluorescence in Situ Hybridization (FISH) Analysis in Human Sperm Cells.- 20 Microdissection and Reverse Chromosome Painting.- 21 Comparative genomic hybridisation (CGH).- VI Techniques in Development.- 22 Fetal Cells in Maternal Blood.- 23 Spectral Karyotyping in Clinical and Tumor Cytogenetics.- 24 Chromosome Analysis by Multiplex-FISH (M-FISH).
