Webb Annual Reports on NMR Spectroscopy

1. Auflage 2009
ISBN: 978-0-08-088508-7
Verlag: Elsevier Science & Techn.
Format: EPUB
Kopierschutz: 6 - ePub Watermark

E-Book, Englisch, 434 Seiten

Nuclear magnetic resonance (NMR) is an analytical tool used by chemists and physicists to study the structure and dynamics of molecules. In recent years, no other technique has gained such significance as NMR spectroscopy. It is used in all branches of science where precise structural determination is required and where the nature of interactions and reactions in solution is being studied. Annual Reports on NMR Spectroscopy has established itself as a premier means for the specialist and non-specialist alike to become familiar with new techniques and applications of NMR spectroscopy.
* Provides updates on the latest developments in NMR spectroscopy
* Includes comprehensive review articles
* Highlights the increasing importance of NMR spectroscopy as a technique for structural determination

Autoren/Hrsg.

Webb, Graham A.

Inhaltsverzeichnis

1;Front Cover;1
2;Annual Reports on NMR Spectroscopy;4
3;Copyright Page;5
4;Contents;6
5;Contributors;10
6;Preface;12
7;Chapter 1: NMR of Antimicrobial Peptides;14
7.1;1. Introduction;15
7.2;2. Mechanism of Action;17
7.3;3. Solution-State NMR;21
7.4;4. Solution-State NMR Experiments;23
7.5;5. Solvent Considerations;26
7.6;6. Structures of Antimicrobial Peptides in Micelles;29
7.7;7. Organic Membrane Mimetic Solvents;32
7.8;8. Orientation in Detergent Micelles;36
7.9;9. Structure Determination of Larger Complexes;39
7.10;10. Endotoxin Binding by Antimicrobial Peptides;42
7.11;11. Recombinant Expression of Antimicrobial Peptides;42
7.12;12. Solid-State NMR of Antimicrobial Peptides;45
7.13;13. Peptide Orientation in Bilayers;46
7.14;14. Depth of Membrane Insertion;50
7.15;15. Oligomerization of Antimicrobial Peptides;51
7.16;16. Bilayer Perturbations;52
7.17;17. Conclusions;57
7.18;Acknowledgments;58
7.19;References;58
8;Chapter 2: NMR Studies of Protein Folding: Folding Studies of Calcium-Binding Lysozyme and alpha-Lactalbumin;66
8.1;1. Introduction;67
8.2;2. Hydrogen-Deuterium Exchange;69
8.3;3. Structure and Dynamics of Unfolded States of Proteins;81
8.4;4. Hydrogen Exchange Pulse Labelling and Real-Time NMR;85
8.5;5. Concluding Remarks;87
8.6;References;87
9;Chapter 3: Applications of the NOE in Molecular Biology;90
9.1;1. Introduction;91
9.2;2. The Basics;91
9.3;3. Faster and Better;102
9.4;4. Complexes;110
9.5;5. Interactions with Water;114
9.6;6. NOEs Involving Nuclei Other than 1H;117
9.7;Abbreviations;118
9.8;Acknowledgment;118
9.9;References;118
10;Chapter 4: Recent Applications of REDOR to Biological Problems;124
10.1;1. Introduction;125
10.2;2. Applications with Peptides;125
10.3;3. Applications with Peptides in Membranes;132
10.4;4. Other Applications;139
10.5;5. Conclusions and Summary of Earlier Applications;147
10.6;Acknowledgments;148
10.7;References;148
11;Chapter 5: Electrophoretic NMR-Ions, Molecules, Mixtures, Pores and Complexes;152
11.1;1. Theoretical and Historical Perspectives;153
11.2;2. Practical Aspects;153
11.3;3. Investigations into Small and Large Molecule Mobilities, and Complex Formation;157
11.4;4. Determination of Small Molecule and Ion Mobility;167
11.5;5. Outlook;170
11.6;References;171
12;Chapter 6: Two-Dimensional Magnetic Resonance Spectroscopy on Biopsy and In Vivo;174
12.1;1. Introduction;175
12.2;2. Assignments;176
12.3;3. Spectral Filtering;180
12.4;4. Analysis of Human Biopsies by COSY;182
12.5;5. Colon;188
12.6;6. Pre-Invasive Ovarian Lesions Identified by COSY;189
12.7;7. In Vivo 2D MRS;192
12.8;8. Human Muscle;195
12.9;9. Human Bone Marrow;197
12.10;10. Prostate;197
12.11;11. Brain;201
12.12;12. Breast;201
12.13;13. 2D Spectral Fitting (ProFit);207
12.14;14. Conclusions;207
12.15;Abbreviations;208
12.16;References;210
13;Subject Index;214