Wassarman Differentiation of Embryonic Stem Cells

1. Auflage 2003
ISBN: 978-0-08-054616-2
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band Volume 365, 509 Seiten, Web PDF

Reihe: Methods in Enzymology

This volume covers all aspects of embryonic stem cell differentiation, including mouse embryonic stem cells, mouse embryonic germ cells, monkey and human embryonic stem cells, and gene discovery.* Early commitment steps and generation of chimeric mice* Differentiation to mesoderm derivatives* Gene discovery by manipulation of mouse embryonic stem cells

Autoren/Hrsg.

Wassarman, Paul

Inhaltsverzeichnis

1;Cover;1
2;Differentiation of Embryonic Stem Cells;4
3;Copyright Page;5
4;Table of Contents;6
5;Contributors to Volume 365;10
6;Preface;16
7;Volumes In Series;18
8;Section I: Differentiation of Mouse Embryonic Stem Cells;48
8.1;Chapter 1. Lineage Specific Differentiation of Mouse ES Cells: Formation and Differentiation of Early Primitive Ectoderm-like (EPL) Cells;50
8.2;Chapter 2. Differentiation of F1 Embryonic Stem Cells into Viable Male and Female Mice by Tetraploid Embryo Complementation;72
8.3;Chapter 3. Hematopoietic Commitment of ES Cells in Culture;86
8.4;Chapter 4. In Vitro Differentiation of Mouse Embryonic Stem Cells: Hematopoietic and Vascular Cell Types;106
8.5;Chapter 5. In Vitro Differentiation of Mouse Embryonic Stem Cells to Hematopoietic Cells on an OP9 Stromal Cell Monolayer;119
8.6;Chapter 6. In Vitro Differentiation of Mouse ES Cells: Hematopoietic and Vascular Development;130
8.7;Chapter 7. In Vitro Differentiation of Mouse ES Cells into Hematopoietic, Endothelial, and Osteoblastic Cell Lineages: The Possibility of In Vitro Organogenesis;145
8.8;Chapter 8. Development of Hematopoietic Repopulating Cells from Embryonic Stem Cells;161
8.9;Chapter 9. The In Vitro Differentiation of Mouse Embryonic Stem Cells into Neutrophils;176
8.10;Chapter 10. Development and Analysis of Megakaryocytes from Murine Embryonic Stem Cells;189
8.11;Chapter 11. Development of Lymphoid Lineages from Embryonic Stem Cells In Vitro;205
8.12;Chapter 12. Probing Dendritic Cell Function by Guiding the Difierentiation of Embryonic Stem Cells;216
8.13;Chapter 13. Gene Targeting Strategies for the Isolation of Hematopoietic and Endothelial Precursors from Differentiated ES Cells;233
8.14;Chapter 14. Establishment of Multipotent Hematopoietic Progenitor Cell Lines from ES Cells Differentiated In Vitro;249
8.15;Chapter 15. Vasculogenesis and Angiogenesis from In Vitro Differentiation of Mouse Embryonic Stem Cells;261
8.16;Chapter 16. ES Cell Differentiation to the Cardiac Lineage;275
8.17;Chapter 17. Bone Nodule Formation via In Vitro Differentiation of Murine Embryonic Stem Cells;288
8.18;Chapter 18. In Vitro Differentiation of Mouse ES Cells: Bone and Cartilage;298
8.19;Chapter 19. Development of Adipocytes from Di.erentiated ES Cells;315
8.20;Chapter 20. In Vitro Differentiation of Embryonic Stem Cells into Hepatocytes;324
8.21;Chapter 21. Differentiation of Mouse Embryonic Stem Cells into Pancreatic and Hepatic Cells;334
8.22;Chapter 22. Generating CNS Neurons from Embryonic, Fetal, and Adult Stem Cells;350
8.23;Chapter 23. Defined Conditions for Neural Commitment and Differentiation;374
8.24;Chapter 24. Development of Melanocytes from ES Cells;388
9;Section II: Differentiation of Mouse Embryonic Germ Cells;398
9.1;Chapter 25. Isolation and Culture of Embryonic Germ Cells;400
10;Section III: Gene Discovery by Manipulation of Mouse Embryonic Stem Cells;412
10.1;Chapter 26. Gene Trap Mutagenesis in Embryonic Stem Cells;414
10.2;Chapter 27. Gene Trap Vector Screen for Developmental Genes in Differentiating ES Cells;433
10.3;Chapter 28. Gene-Based Chemical Mutagenesis in Mouse Embryonic Stem Cells;453
11;Section IV: Differentiation of Monkey and Human Embryonic Stem Cells;464
11.1;Chapter 29. Growth and Differentiation of Cynomolgus Monkey ES Cells;466
11.2;Chapter 30. Isolation, Characterization, and Differentiation of Human Embryonic Stem Cells;476
11.3;Chapter 31. Factors Controlling Human Embryonic Stem Cell Differentiation;493
11.4;Chapter 32. Development of Cardiomyocytes from Human ES Cells;508
12;Author Index;522
13;Subject Index;548
14;Color Plate Section;558