Vigetti / Theocharis The Extracellular Matrix

Methods and Protocols
Erscheinungsjahr 2019
ISBN: 978-1-4939-9133-4
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1952, 278 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume details established experimental protocols on the isolation, characterization, and detection of extracellular matrix (ECM) molecules as well as methods to study the activity and role of ECM components on various biological functions, the formation of exosomes, and tissue surrogates. Chapters display a variety of protocols ranging from biochemical, cell, and molecular biology assays to complex tissue imaging techniques and in vivo models to elucidate the role of the extracellular matrix. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and cutting-edge, The Extracellular Matrix: Methods and Protocols aims to provide protocols and applications that can be used by both Ph.D. students and senior researchers to advance research in the field of extracellular matrix biology.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Vigetti, Davide

Theocharis, Achilleas D.

Inhaltsverzeichnis

The Complex Interplay between Extracellular Matrix and Cells in Tissues.- Method for Studying ECM Expression: In Situ RT-PCR.- Visualizing the Supramolecular Assembly of Collagen.- Steady-state and Pulse-chase Analyses of Fibrillar Collagen.- Methods for Isolation and Characterization of Sulfated Glycosaminoglycans from Marine Invertebrates.- Analysis of Proteoglycan Synthesis and Secretion in Cell Culture Systems.- Analysis of UDP-Sugars from Cultured Cells and Small Tissue Samples.- Methods for Hyaluronan Molecular Mass Determination by Agarose Gel Electrophoresis.- Hyaluronan Pericellular Matrix: Particle Exclusion Assay.- Determination of Cell Surface Hyaluronan through Flow Cytometry.- Method for Detecting Hyaluronan in Isolated Myenteric Plexus Ganglia of Adult Rat Small Intestine.- Analyzing Hyaluronidases in Biological Fluids.- Method for Studying the Physical Effect of Extracellular Matrix on Voltage-dependent IonChannels Gating.- Methods for Monitoring Matrix-Induced Autophagy.- Method for Determining Gelatinolytic Activity in Tissue: In Situ Gelatin Zymography.- Method for Determining Gelatinolytic Activity in Tissue Extracts: Real-Time Gelatin Zymography.- In Vitro Spheroid Sprouting Assay of Angiogenesis.- Matrigel Plug Assay for In Vivo Evaluation of Angiogenesis.- Exosomes from Cell Culture Conditioned Medium: Isolation by Ultracentrifugation and Characterization.- Preparation and Characterisation of Rich in Extracellular Matrix Tissue Surrogates Using the Principles of Macromolecular Crowding.- Novel Approaches for Extracellular Matrix Targeting in Disease Treatment.