Velmurugu | Dynamics and Mechanism of DNA-Bending Proteins in Binding Site Recognition | Buch | 978-3-319-45128-2 | sack.de

Buch, Englisch, 199 Seiten, Format (B × H): 160 mm x 241 mm, Gewicht: 4616 g

Reihe: Springer Theses

Velmurugu

Dynamics and Mechanism of DNA-Bending Proteins in Binding Site Recognition


1. Auflage 2017
ISBN: 978-3-319-45128-2
Verlag: Springer International Publishing

Buch, Englisch, 199 Seiten, Format (B × H): 160 mm x 241 mm, Gewicht: 4616 g

Reihe: Springer Theses

ISBN: 978-3-319-45128-2
Verlag: Springer International Publishing


Using a novel approach that combines high temporal resolution of the laser T-jump technique with unique sets of fluorescent probes, this study unveils previously unresolved DNA dynamics during search and recognition by an architectural DNA bending protein and two DNA damage recognition proteins.

Many cellular processes involve special proteins that bind to specific DNA sites with high affinity.  How these proteins recognize their sites while rapidly searching amidst ~3 billion nonspecific sites in genomic DNA remains an outstanding puzzle. Structural studies show that proteins severely deform DNA at specific sites and indicate that DNA deformability is a key factor in site-specific recognition. However, the dynamics of DNA deformations have been difficult to capture, thus obscuring our understanding of recognition mechanisms. 

The experiments presented in this thesis uncover, for the first time, rapid (~100-500 microseconds) DNA unwinding/bending attributed to nonspecific interrogation, prior to slower (~5-50 milliseconds) DNA kinking/bending/nucleotide-flipping during recognition. These results help illuminate how a searching protein interrogates DNA deformability and eventually “stumbles” upon its target site. Submillisecond interrogation may promote preferential stalling of the rapidly scanning protein at cognate sites, thus enabling site-recognition.  Such multi-step search-interrogation-recognition processes through dynamic conformational changes may well be common to the recognition mechanisms for diverse DNA-binding proteins. 

Velmurugu Dynamics and Mechanism of DNA-Bending Proteins in Binding Site Recognition jetzt bestellen!

Zielgruppe


Research


Autoren/Hrsg.


Weitere Infos & Material


Introduction.- Methods.- Integration Host Factor (IHF)-DNA interaction.- Lesion Recognition by Xeroderma Pigmentosum C (XPC) Protein.- DNA Mismatch Repair.


Yogambigai Velmurugu was awarded the PhD degree by the University of Illinois, Chicago, in 2015. 



Ihre Fragen, Wünsche oder Anmerkungen
Vorname*
Nachname*
Ihre E-Mail-Adresse*
Kundennr.
Ihre Nachricht*
Lediglich mit * gekennzeichnete Felder sind Pflichtfelder.
Wenn Sie die im Kontaktformular eingegebenen Daten durch Klick auf den nachfolgenden Button übersenden, erklären Sie sich damit einverstanden, dass wir Ihr Angaben für die Beantwortung Ihrer Anfrage verwenden. Selbstverständlich werden Ihre Daten vertraulich behandelt und nicht an Dritte weitergegeben. Sie können der Verwendung Ihrer Daten jederzeit widersprechen. Das Datenhandling bei Sack Fachmedien erklären wir Ihnen in unserer Datenschutzerklärung.