Buch, Englisch, 403 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 7844 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, 403 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 7844 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-4939-4371-5
Verlag: Springer
Infection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) impact millions of lives and cause tens of thousands of mortalities each year. Dengue is a global public health emergency especially since there is no preventative vaccine or antiviral treatment for dengue disease. Dengue: Methods and Protocols offers the increasing number of dengue researchers a one-stop protocol book with techniques compiled from the leading laboratories working on dengue. Chapters cover topics such as dengue virus isolation from clinical samples, quantification of human antibodies against the virus, assays to quantify the virus particles, the widely used mouse model to study dengue pathogenesis, vaccine and antiviral efficacies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Dengue: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies on dengue research.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
- Naturwissenschaften Biowissenschaften Virologie
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Medizinische Mikrobiologie & Virologie
- Naturwissenschaften Biowissenschaften Enzymologie
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Infektionskrankheiten
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Umweltmedizin, Arbeitsmedizin, Tropenmedizin, Sportmedizin Reisemedizin, Tropenmedizin
Weitere Infos & Material
Part I. Modern Quantitative Virological Methods
1. Dengue Virus Growth, Purification and Fluorescent Labeling
Summer Zhang, Kuan Rong Chan, Hwee Cheng Tan, and Eng Eong Ooi
2. Isolation and Titration of Dengue Viruses by the Mosquito Inoculation Technique
Milly M. Choy and Duane J. Gubler
3. Measuring Antibody Neutralization of Dengue Virus (DENV) Using a Flow Cytometry-Based Technique
A. Ruklanthi de Alwis and Aravinda M. de Silva
4. Dengue Virus Purification and Sample Preparation for Cryo-Electron Microscopy
Joanne L. Tan and Lok Shee Mei
5. Development of a Multiplex Bead-Based Assay to Monitor Dengue Virus Seroconversion
Kaiting Ng and John E. Connolly
6. Pseudo-Infectious Reporter Virus Particles for Measuring Antibody-Mediated
Neutralization and Enhancement of Dengue Virus Infection
Swati Mukherjee, Ted Pierson and Kimberly A. Dowd
7. Cell-Based Flavivirus Infection (CFI) Assay for the Evaluation of Dengue Antiviral Candidates Using High Content Imaging
Kah Hin Tan, Kitti Chan Wing Ki, Satoru Watanabe, Subhash G. Vasudevan and Manoj Krishnan
Part II. Reverse Genetic Systems to Study Virus Replication and Evolution
8. Development and Application of Dengue Virus Reverse Genetic Systems
Andrew Davidson
9. DENV4 Renilla Luciferase Reporter Replicons
Sofia Alcaraz-Estrada, Rosa Del Angel, and R. Padmanabhan
10. Targeted Mutagenesis of Dengue Virus Type 2 replicon RNA by Yeast in vivo
Recombination
Mark Manzano and R. Padmanabhan
11. Identification of Dengue-Specific Human Antibody Fragments Using Phage Display
Moon Y.F. Tay, Chin Chin Lee, Subhash G. Vasudevan and Nicole J. Moreland
12. Next Generation Whole Genome Sequencing of Dengue Virus
Pauline Poh Kim Aw, Paola Florez de Sessions, Andreas Wilm, Long Truong Hoang, Niranjan Nagarajan, October M. Sessions, and Martin Lloyd Hibberd
Part III. Modern Methods to Study Conformation of RNA Proteins and Their Molecular Interactions
13. MPGAfold in Dengue Secondary Structure Prediction
Wojciech K. Kasprzak and Bruce A. Shapiro
14. Insights into Secondary and Tertiary Interactions of Dengue Virus RNA by SHAPE
Joanna Sztuba-Solinska and Stuart F.J. Le Grice
15. Use of Small Angle X-Ray Scattering to Investigate the Structure and Function of Dengue Virus NS3 and NS5
Kyung H. Choi and Marc Morais
16. Identification of Dengue RNA Binding Proteins Using RNA Chromatography and Quantitative Mass Spectrometry
A.M. Ward, J. Gunaratne, M. A. Garcia-Blanco
17. Analysis of Affinity of Dengue Virus Protein Interaction Using BIAcore
Yau Yin Hoe and Susana Geifman Shochat
18. Functional Genomics Approach for the Identification of Human Host Factors Supporting Dengue Viral Propagation
Nicholas J. Barrows, Sharon F. Jamison, Shelton S. Bradrick, Caroline Le Sommer, So Young Kim, James Pearson, and Mariano A. Garcia-Blanco
19. Investigating Dengue Virus Non-Structural Protein 5 (NS5) Nuclear Import
Johanna E. Fraser, Stephen M. Rawlinson, Chunxiao Wang, David A. Jans, and Kylie M. Wagstaff
Part IV. In vitro Enzyme Assays for Antiviral Screening
20. Small Molecule Inhibitor Discovery for Dengue Virus Protease Using High Throughput Screening
Mark Manzano, Janak Padia, and R. Padmanabhan
21. Construction of Dengue Virus Protease Expression Plasmid and in vitro Protease Assay for Screening Antiviral Inhibitors
Huiguo Lai, Tadahisa Teramoto and Radhakrishnan Padmanabhan
22. Construction of Plasmid, Bacterial Expression, Purification, and Assay of Dengue Virus Type 2 NS5 Methyltransferase
Siwaporn Boonyasuppayakorn and Radhakrishnan Padmanabhan
Part V. Dengue Animal Model for Vaccines and Antivirals
23. Animal Models in Dengue
Emily Plummer and Sujan Shresta
24. Evaluation of Dengue Antiviral Candidates In Vivo in Mouse Model
Satoru Watanabe and Subhash G. Vasudevan
