Uversky / Longhi Instrumental Analysis of Intrinsically Disordered Proteins

Assessing Structure and Conformation
1. Auflage 2011
ISBN: 978-0-470-60260-7
Verlag: John Wiley & Sons
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 736 Seiten, E-Book

Reihe: Wiley Series in Protein and Peptide Science

Instrumental techniques for analyzing intrinsically disorderedproteins
The recently recognized phenomenon of protein intrinsic disorderis gaining significant interest among researchers, especially asthe number of proteins and protein domains that have been shown tobe intrinsically disordered rapidly grows. The first reference totackle this little-documented area, Instrumental Analysis ofIntrinsically Disordered Proteins: Assessing Structure andConformation provides researchers with a much-needed,comprehensive summary of recent achievements in the methods forstructural characterization of intrinsically disordered proteins(IDPs).
Chapters discuss:
* Assessment of IDPs in the living cell
* Spectroscopic techniques for the analysis of IDPs, including NMRand EPR spectroscopies, FTIR, circular dichroism, fluorescencespectroscopy, vibrational methods, and single-molecule analysis
* Single-molecule techniques applied to the study of IDPs
* Assessment of IDP size and shape
* Tools for the analysis of IDP conformational stability
* Mass spectrometry
* Approaches for expression and purification of IDPs
With contributions from an international selection of leadingresearchers, Instrumental Analysis of Intrinsically DisorderedProteins: Assessing Structure and Conformation fills animportant need in a rapidly growing field. It is required readingfor biochemists, biophysicists, molecular biologists, geneticists,cell biologists, physiologists, and specialists in drug design anddevelopment, proteomics, and molecular medicine with an interest inproteins and peptides.

Autoren/Hrsg.

Uversky, Vladimir

Longhi, Sonia

Über Autor(innen)

Vladimir N. Uversky, PhD, is a Senior Research Professor atthe Indiana University School of Medicine. He obtained his academicdegrees from Moscow Institute of Physics and Technology (PhD) andthe Institute of Theoretical and Experimental Biophysics, RussianAcademy of Sciences. Professor Uversky has authored over 300scientific publications and edited several books and book series onprotein structure, function, folding, and misfolding. He is also aneditor of several scientific journals.
Sonia Longhi, PhD, is a Director of Research at theCenter for the National Scientific Research (CNRS), heading theStructural Disorder and Molecular Recognition group within the AFMBlaboratory. She obtained her PhD from the Università degliStudi of Milan in 1993. She has authored more than sixty scientificpublications and edited a book on measles virus nucleoprotein.