Uttamchandani / Yao Small Molecule Microarrays

Methods and Protocols
2010
ISBN: 978-1-60761-845-4
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 669, 228 Seiten, eBook

Reihe: Methods in Molecular Biology

Small molecule microarrays (SMM) were introduced just a decade ago in 1999 and, within a short space of time, have already established themselves as a vibrant, next generation platform for high-throughput screening. Small Molecule Microarrays: Methods and Protocols showcases a collection of contributions guiding researchers toward ways in which small molecule microarray technology may be deployed for multiplexed screening and profiling. Organized by the categories of small molecules presented on the microarrays, this detailed volume describes in-depth techniques for chemical libraries, peptide libraries, and carbohydrate microarrays. Written in the highly successful Methods in Molecular Biology™ series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, vital tips on troubleshooting this often difficult technology, and advice on avoiding known pitfalls.

Authoritative and cutting-edge, Small Molecule Microarrays: Methods and Protocols provides meticulous depictions of key hands-on experience and seeks to inspire a future generation of microarray practitioners to take this significant technology forward.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Uttamchandani, Mahesh

Yao, Shao Q.

Inhaltsverzeichnis

1. The Expanding World of Small Molecule Microarrays Mahesh Uttamchandani and Shao Q. Yao

2. Construction of Photo-Cross-Linked Microarrays of Small Molecules Naoki Kanoh

3. Small Molecule Microarray: Functional-Group Specific Immobilization of Small Molecules Hyang Yeon Lee and Seung Bum Park

4. Detecting Protein-Small Molecule Interactions Using Fluorous Small-Molecule Microarrays Arturo J. Vegas and Angela N. Koehler

5. A Method for Small Molecule Microarray (SMM)-Based Screening for the Rapid Discovery of Affinity-Based Probes (AfBPs) Haibin Shi, Mahesh Uttamchandani, and Shao Q. Yao

6. Nanodroplet Chemical Microarrays and Label-Free Assays Dhaval Gosalia and Scott L. Diamond

7. Nanodroplet Microarrays for High-Throughput Enzyme Screening Kang L.D. Aw, Shao Q. Yao, and Mahesh Uttamchandani

8. The Application of the Chemical Array for Biological Study Isao Miyazaki, Siro Simizu, and Hiroyuki Osada

9. Peptide Arrays with a Chip Alexander Nesterov, Edgar Dörsam, Yun-Chien Cheng, Christopher Schirwitz, Frieder Märkle, Felix Löffler, Kai König, Volker Stadler, Ralf Bischoff, and Frank Breitling

10. In situ Chemical Modification of Peptide Microarrays: Characterization by Desorption/Ionization on Silicon Nanowires Gaëlle Piret, Hervé Drobecq, Rabah Boukherroub, and Oleg Melnyk

11. In situ Chemical Modification of Peptide Microarrays: Application to the Study of the Antibody Responses to Methylated Antigens Rémi Desmet, Eric Diesis, Hervé Drobecq, Carine Rouanet, Karim Chemlal, Anne-Sophie Debrie, Jean-Michel Hougardy, Françoise Mascart, Camille Locht, and Oleg Melnyk

12. Peptide Microarrays on Coated Silicon Slides for Highly Sensitive Antibody Detection Marina Cretich, Francesco Damin, Renato Longhi, Cecilia Gotti, Clelia Galati, Lucio Renna, and Marcella Chiari

13. Antibody Signatures Defined by High-Content Peptide Microarray Analysis Antonia Masch, Johannes Zerweck, Ulf Reimer, Holger Wenschuh, and Mike Schutkowski

14. HighDensity Peptide Microarrays for Proteome-Wide Fingerprinting of Kinase Activities in Cell Lysates Alexandra Thiele, Matthias Weiwad, Johannes Zerweck, Gunter Fischer, and Mike Schutkowski

15. A Peptide Microarray for Detecting Protein Kinase Activity in Cell Lysates Xiaoming Han and Yoshiki Katayama

16. Chemical Microarrays Constructed by Selective Attachment of Hydrazide-Conjugated Substances to Epoxide Surfaces and Their Applications Sungjin Park, Myung-Ryul Lee, and Injae Shin

17. A Microarray-Based Method to Perform Nucleic Acid Selections Olga Aminova and Matthew D. Disney