Uppu / Murthy / Pryor Free Radicals and Antioxidant Protocols

2. Auflage 2010
ISBN: 978-1-60327-029-8
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 610, 480 Seiten, eBook

Reihe: Methods in Molecular Biology

Zielgruppe

Research

Autoren/Hrsg.

Uppu, Rao M.

Murthy, Subramanyam N.

Pryor, William A.

Parinandi, Narasimham L.

Inhaltsverzeichnis

Contents

Preface

Contributors

Part I Reactive Oxygen and Nitrogen Species

1. In Vivo Imaging of Free Radicals and Oxygen
Deepti S. Vikram, Brian K. Rivera, and Periannan Kuppusamy

2 . In Vivo Measurement of Tissue Oxygen using Electron Paramagnetic Resonance Spectroscopy with Oxygen-sensitive Paramagnetic Particle, Lithium Phthalocyanine

F. Hyodo, S. Matsumoto, E. Hyodo, A. Matsumoto, K. Matsumoto, and M. C. Krishna

3. Measurement of Plasma Nitrite by Chemiluminescence

Enika Nagababu and Joseph M Rifkind

4. Determination of Glutathione, Mitochondrial Transmembrane Potential, and Cytotoxicity in H9c2 Cardiomyoblasts Exposed to Reactive Oxygen and Nitrogen Species
K. Sathishkumar, Xueli Gao, Achuthan C. Raghavamenon, Narasimham L. Parinandi, and Rao M. Uppu

Part II Natural and Synthetic Antioxidants

5. Phenolic Acids and Flavonoids: Ocurrence and Analytical Methods
Constantine D. Stalikas

6. Design, Synthesis, and Action of Antiatherogenic Antioxidants
Osamu Cynshi, Kunio Tamura, and Etsuo Niki

7. Preparation of Drug-Loaded Polymeric Nanoparticles and Evaluation of the Antioxidant Activity against Lipid Peroxidation

Adriana R. Pohlmann, Scheila Rezende Schaffazick, Tânia B. Creczynski-Pasa, and Sílvia S. Guterres

8. Nanoparticle and Iron Chelators as a Potential Novel Alzheimer Therapy
Gang Liu, Ping Men, George Perry, and Mark A. Smith

9. Synthesis and Characterization of Polymer Nanocarriers for the Targeted Delivery of Therapeutic Enzymes

Eric Simone, Thomas Dziubla , Vladimir Shuvaev, and Vladimir R. Muzykantov

10. Assessment of Antioxidant Activity of Eugenol In Vitro and In Vivo

Enika Nagababu, Joseph M. Rifkind, Sesikeran Boindala, andLakshmaiah Nakka

Part III Cellular Oxidative Stress

11. The Generation of Stable Oxidative Stress-Resistant Phenotypes in Chinese Hamster Fibroblasts Chronically Exposed to Hydrogen Peroxide or Hyperoxia
Douglas R. Spitz and Shannon J. Sullivan

12. A Simple Method for Effective and Safe Removal of Membrane Cholesterol from Lipid Rafts in Vascular Endothelial Cells: Implications in Oxidant-Mediated Lipid Signaling
Michelle A. Kline, E. S. O’Connor Butler, Adam Hinzey, Sean Sliman, Clay B. Marsh, Rao M. Uppu, and Narasimham L. Parinandi

13. Superoxide Dismutase - A Target for Gene Therapeutic Approach to Reduce Oxidative Stress in Erectile Dysfunction
W. Deng , T.J. Bivalacqua , W. Hellstrom , S. N. Murthy , and Philip J. Kadowitz

14. Assessing the Reductive Capacity of Cells by Measuring the Recycling of Ascorbic and Lipoic Acids
James M. May

15. Biomarkers of Oxidative Stress: Methods and Measures of Oxidative DNA Damage (COMET Assay) and Telomere Shortening
Muthuswamy Balasubramanyam, Antonysunil Adaikalakoteswari, Zaheer Sameermahmood, and Viswanathan Mohan

16. Simultaneous Analysis of Mutiple Redox-Sensitive and Apoptotic Gene Expression in Hypothalamic Neurons Exposed to Cholesterol Ozonation Products
K.Sathishkumar, Achuthan C. Raghavamenon, Karunakaran Ganeshkumar, Rameshwari Telaprolu and Rao M. Uppu

17. Redox Homeostasis and Cellular Stress Response in Aging and Neurodegeneration
Vittorio Calabrese, Cesare Mancuso, Riccardo Lentile, A.M. Giuffrida Stella, and D. Allan Butterfield.

18. Gene Therapy Techniques for the Delivery of Endothelial Nitric Oxide Synthase to the Lung for Pulmonary Hypertension
W. Deng, T.J. Bivalacqua, W. Hellstrom, Subramanyam N. Murthy, and Philip J. Kadowitz

Part IV DNA Oxidation,