The Resolution Revolution: Recent Advances in Cryoem

Erscheinungsjahr 2016
ISBN: 978-0-12-805382-9
Verlag: ACADEMIC PR INC

Buch, Englisch, Band 579, 486 Seiten, Format (B × H): 152 mm x 231 mm, Gewicht: 916 g

Reihe: Methods in Enzymology

cryoEM, a new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods and new developments in recording images, the creation, evaluation and validation of 3D maps from the images, model building into maps and refinement of the resulting atomic structures, and applications of essentially single particle methods to helical structures and to sub-tomogram averaging.

Autoren/Hrsg.

Weitere Mitwirkende

Crowther, R. A.

Crowther, R. A

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Enzymologie

Inhaltsverzeichnis

1. Direct Electron Detectors - Methods in Enzymology (2016) Greg McMullan, Abdul R. Faruqi and Richard Henderson 2. Specimen Behavior in the Electron Beam Robert M. Glaeser 3. Specimen Preparation for High-Resolution Cryo-EM Christopher J. Russo and Lori A. Passmore 4. Strategies for Automated Cryoem Data Collection Using Direct Detectors Anchi Cheng, Yong Zi Tan, Venkat Dandey, Clinton S. Potter and Bridget Carragher 5. Processing of Cryo-EM Movie Data Zev A. Ripstein and John L. Rubinstein 6. Processing of Structurally Heterogeneous Cryo-EM Data in RELION Sjors H.W. Scheres 7. Single Particle Refinement and Variability Analysis in EMAN2.1 Steven J. Ludtke 8. Frealign: An Exploratory Tool for Single Particle Cryo-EM Nikolaus Grigorieff 9. Testing the Validity of Single Particle Maps at Low and High Resolution Peter B. Rosenthal 10. Tools for Model Building and Optimization into Near Atomic Resolution Electron Cryo-Microscopy Density Maps Frank DiMaio and Wah Chiu 11. Refinement of Atomic Structures Against CryoEM Maps Garib N. Murshudov 12. Cryo-EM Structure Determination Using Segmented Helical Image Reconstruction Simon A. Fromm and Carsten Sachse 13. Cryo-Electron Tomography and Subtomogram Averaging William Wan and John A.G. Briggs

Über Autor(innen)

Crowther, R. A
Tony Crowther is an emeritus scientist at the MRC Laboratory of Molecular Biology in Cambridge, where he was joint head of the Structural Studies Division from 1994 to 2005. He has been involved in electron microscopy and image processing of biological structures for many years. His particular biological interests have been in viruses such as hepatitis B and in the abnormal filaments that form in neurodegenerative diseases. He is a Fellow of the Royal Society of London, a Fellow of the Academy of Medical Sciences and a member of the European Molecular Biology Organization.