Tharakan Traumatic and Ischemic Injury

Methods and Protocols
Erscheinungsjahr 2018
ISBN: 978-1-4939-7526-6
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 245 Seiten

Reihe: Springer Protocols

This volume focuses on procedures for the development and application of several research animal models and in vitro methods that allow researchers to gain insight into the underlying cellular, biochemical, and physiological mechanisms involved in traumatic and ischemic injury. The chapters in this book discuss topics, such as animal models and techniques commonly used to study traumatic brain injury, hemorrhagic shock, ischemic and hemorrhagic strokes, sepsis, burn injury, hind limb ischemia, myocardial-ischemia-reperfusion injury, intracranial pressure, global hypoxia-induced perinatal seizures models, and in vitro models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Traumatic and Ischemic Injury: Methods and Protocols is a valuable resource for novices with limited experience to help them initiate new research projects, and established researchers to help them identify comparable approaches and strategies to their studies in this field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Tharakan, Binu

Inhaltsverzeichnis

Mouse Injury Model of Polytrauma and Shock.- Measurement of Intracranial Pressure in Freely Moving Rats.- A Lateral Fluid Percussion Injury Model for Studying Traumatic Brain Injury in Rats.- A Mouse Controlled Cortical Impact Model of Traumatic Brain Injury for Studying Blood-Brain Barrier Dysfunctions.- A Rat Model of Hemorrhagic Shock for Studying Vascular Hyperpermeability.- Assessment of Cardiovascular Function and Microvascular Permeability in a Conscious Rat Model of Alcohol Intoxication Combined With Hemorrhagic Shock and Resuscitation.- Intracerebral Hemorrhage in Mice.- A Rat Burn Injury Model for Studying Changes in Microvascular Permeability.- Modeling Transient Focal Ischemic Stroke in Rodents by Intraluminal Filament Method of Middle Cerebral Artery Occlusion.- A Complete Guide to Using the Endothelin-1 Model of Stroke in Conscious Rats for Acute and Long Term Recovery Studies.- A Murine Model of Hind Limb Ischemia to Study Angiogenesis and Arteriogenesis.- A MurineModel of Myocardial Ischemia-Reperfusion Injury.- A Rat Model of Perinatal Seizures Provoked by Global Hypoxia.- Experimental Protocol for Cecal Ligation and Puncture Model of Polymicrobial Sepsis and Assessment of Vascular Functions in Mice.- Methods to Study the Innate Immune Response to Sepsis.- An Ovine Model for Studying the Pathophysiology of Septic Acute Kidney Injury.- An In Vitro Model of Traumatic Brain Injury.- An In Vitro Oxygen-Glucose Deprivation Model for Studying Ischemia-Reperfusion Injury of Neuronal Cells.- Measurement of Microvascular Endothelial Barrier Dysfunction and Hyperpermeability In Vitro.