Sztefko Immunodiagnostics and Patient Safety

1. Auflage 2011
ISBN: 978-3-11-024948-4
Verlag: De Gruyter
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 3, 172 Seiten, Format (B × H): 170 mm x 240 mm

Reihe: Patient Safety

Today most of immunochemistry methods for the determination of proteins, peptides, drugs, and many small molecules are fully automated, with good precision, excellent sensitivity and short reaction time. However, inaccuracy due to poor standardization and the presence of interfering substances in biological samples is still a serious and life-threatening issue. Proper validation of methods and quality assurance have little effect on frequency of occurrence of false positive or false negative results, which, if unrecognized, may lead to patient's misdiagnosis, unnecessary treatment or even unnecessary surgery. Deep knowledge of basic principles of immunochemical methods (antigen-antibody reaction, standardization, matrix effect, limit of detection, cross-reactivity, etc.), sources of analyte-independent interferences (preanalitycal errors, the presence of binding proteins, the presence of autoantibodies) and analyte-dependent interferences (presence of heterophilic antibodies, high-dose effect) are very important to understand, detect, reduce and/or eliminate the interferences. This book helps to reduce false results and, at the same time, improve patient's care and patient's safety.

Zielgruppe

Laboratory Professionals, Clinical Chemists

Autoren/Hrsg.

Sztefko, Krystyna

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Immunologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Labormedizin

Inhaltsverzeichnis

Immunodiagnostics and patient safety Table of contents
1. Overview of immunochemistry (basic principles, standardization, limit of detection, cross-reactivity, ect)
2. Interferences in immunochemistry
a. Matrix effects
b. Preanalitycal factors
c. Binding proteins
d. Macroforms of proteins
e. High-dose effect
f. Heterophilic antibody
g. Anti-animal antibody
h. Autoantibody
3. Methods of detection and reduction of interferences

4. The impact of interferences on patients safety - examples

Über Autor(innen)

Krystyna Sztefko, Jagiellonian University, Krakow, Poland.