Staton / Lewis / Bicknell Angiogenesis Assays

A Critical Appraisal of Current Techniques
1. Auflage 2007
ISBN: 978-0-470-02934-3
Verlag: John Wiley & Sons
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 410 Seiten, E-Book

Angiogenesis, the development of new blood vessels from theexisting vasculature, is essential for physiological growth andover 18,000 research articles have been published describing therole of angiogenesis in over 70 different diseases, includingcancer, diabetic retinopathy, rheumatoid arthritis and psoriasis.One of the most important technical challenges in such studies hasbeen finding suitable methods for assessing the effects ofregulators of eh angiogenic response. While increasing numbers ofangiogenesis assays are being described both in vitro and in vivo,it is often still necessary to use a combination of assays toidentify the cellular and molecular events in angiogenesis and thefull range of effects of a given test protein. Although theendothelial cell - its migration, proliferation, differentiationand structural rearrangement - is central to the angiogenicprocess, it is not the only cell type involved. the supportingcells, the extracellular matrix and the circulating blood with itscellular and humoral components also contribute.
In this book, experts in the use of a diverse range of assaysoutline key components of these and give a critical appraisal oftheir strengths and weaknesses. Examples include assays for theproliferation, migration and differentiation of endothelial cellsin vitro, vessel outgrowth from organ cultures, assessment ofendothelial and mural cell interactions, and such in vivo assays asthe chick chorioallantoic membrane, zebrafish, corneal, chamber andtumour angiogenesis models. These are followed by a criticalanalysis of the biological end-points currently being used inclinical trials to assess the clinical efficacy of anti-angiogenicdrugs, which leads into a discussion of the direction futurestudies should take.
This valuable book is of interest to research scientistscurrently working on angiogenesis in both the academic communityand in the biotechnology and pharmaceutical industries. Relevantdisciplines include cell and molecular biology, oncology,cardiovascular research, biotechnology, pharmacology, pathology andphysiology.

Autoren/Hrsg.

Staton, Carolyn

Lewis, Claire

Bicknell, Roy

Inhaltsverzeichnis

Preface.
List of Contributors.
1 Endothelial cell biology(Femke Hillen, VeerleMelotte, Judy R. van Beijnum and Arjan W. Griffioen).
2 Endothelial cell proliferation assays (Wen-SenLee).
3 Endothelial cell migration assays (ChristosPolytarchou, Maria Hatziapostolou and EvangeliaPapadimitriou).
4 Tubule formation assays (Ewen J. Smith and CarolynA. Staton).
5 Modelling the effects of the haemodynamic environment onendothelial cell responses relevant to angiogenesis (GerardB. Nash and Stuart Egginton).
6 Whole or partial vessel outgrowth assays (Cindy H.Chau and William D. Figg).
7 Assaying endothelial-mural cell interactions(Melissa K. Nix and Karen K. Hirschi).
8 Assays for membrane and intracellular signalling events(Vittorio Tomasi, Cristiana Griffoni, Spartaco Santi, PatriziaLenaz, Rosa Anna Iorio, Antonio Strillaci and Enzo Spisni).
9 Implantation of sponges and polymers (Silvia P.Andrade, Monica A. N. D. Ferreira and Tai-Ping Fan).
10 Angiogenesis assays in the chick (Andries Zijlstra,David Mikolon and Dwayne G. Stupack).
11 Corneal angiogenesis assay (Siqing Shan and Mark W.Dewhirst).
12 Dorsal air sac model (Sei Yonezawa, Tomohiro Asaiand Naoto Oku).
13 Chamber assays (Michael D. Menger, Matthias W.Laschke and Brigitte Vollmar).
14 Tumour models: analysis of angiogenesis in vivo(Sven A. Lang, M.D. and Oliver Stoeltzing, M.D).
15 Angiomouse: imageable models of angiogenesis(Robert M. Hoffman).
16 Techniques and advances in vascular imagingin Danio rerio (Kenna R. Mills Shaw and Brant. M.Weinstein).
17 Biological and clinical implications of recruitmentof stem cells into angiogenesis (Gianluigi Castoldi, AntonioCuneo and Gian Matteo Rigolin).
18 Methods for monitoring of the anti-angiogenic activity ofagents in patients: novel trial design (Shannon Smiley,Michael K. K. Wong and Shaker A. Mousa).
19 An overview of current angiogenesis assays: Choice ofassay, precautions in interpretation, future requirements anddirections (Robert Auerbach).
Index.

Über Autor(innen)

Dr CA Staton. Lecturer in Angiogenesis Research, AcademicUnit of Surgical Oncology, University of Sheffield Medical School.
Professor Claire Lewis. Professor of Molecular &Cellular Pathology, Academic Unit of Pathology, University ofSheffield Medical School.
Professor Roy Bicknell. CRUK Molecular Angiogenesis Labs,Institute of Molecular Medicine, University of Oxford.