Buch, Englisch, 379 Seiten, Format (B × H): 178 mm x 254 mm, Gewicht: 735 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, 379 Seiten, Format (B × H): 178 mm x 254 mm, Gewicht: 735 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-2271-1
Verlag: Springer
This volume details cutting-edge methods and protocols for the development, characterization, and applications of multienzyme assemblies. Chapters guide readers through up-to-date techniques applied for the development and emerging applications of multi-enzymatic systems for biotransformations, biosensing, molecular-scale diagnostics and bioelectronics. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Multienzymatic Assemblies: Methods and Protocols aims to be useful for academics and industry professionals working in the field of biotechnology, biochemistry, chemical and biological engineering, nanobiotechnology, and biocatalysis.
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Weitere Infos & Material
1. Recombinant Expression and Purification of Large Bacterial Multienzyme Assemblies for Biosynthetic Processes
James Christenson
2. Multi-Dimensional Fluorescence Live-Cell Imaging for Glucosome Dynamics in Living Human Cells
Songon An, Prakash Parajuli, Erin L. Kennedy, and Minjoung Kyoung3. Mechanisms and Effects of Substrate Channelling in Enzymatic Cascades
Svyatoslav Kondrat and Eric Von Lieres
4. Structural Characterization of Multienzyme Assemblies: An Overview
Anastassios C. Papageorgiou
5. Assessing Protein Interactions for Clustering of Mitochondrial Urea Cycle Enzymes
Ljubica Caldovic, Shivaprasad Bhuvanendran, and Jyoti Jaiswal.
6. DNA Nano-Scaffolds for Multienzyme Systems Assembly
Sung Won Oh, Zhicheng Wang, and Jinglin Fu
7. Strategies for Multienzyme Assemblies
Qing Sun, Maryam Raeeszadeh-Sarmazdeh, Shen-Long Tsai, and Wilfred Chen
8. Random and Positional Immobilization of Multi-Enzyme SystemsHassan Maleki, Kamyar Khoshnevisan, and Hadi Baharifar
9. Compartmentalized Immobilization of Multi-Enzyme Systems
Hadi Baharifar, Kamyar Khoshnevisan, and Hassan Maleki
10. 3D Printed Polylactic Acid Well-Plate for Multi-Enzyme Immobilization
Elena Gkantzou, Anastasia Skonta, Andreas-Georgios Vasios, Haralambos Stamatis
11. Rational Design of Self-Assembling Supramolecular Protein Nanostructures Utilizing The Cucurbit[8]Uril Macrocyclic Host
Elisavet Ioannou and Nikolaos E. Labrou12. Lectin-Mediated Coimmobilization of Cascade Glycoenzymes
Yifei Zhang, Yunzhenshan Gao, and You Yong
13. Self-Assembled Multienzyme Nanostructures for Biocatalysis In Cellulo
Qixin Wei and Jiang Xia
14. Nanoarmored Multi-Enzyme Cascade Catalysis
Mansi Malhotra, Ankarao Kalluri, and Challa Vijaya Kumar
15. Implementing Multi-Enzyme Biocatalytic Systems Using Nanoparticle Scaffolds
Joyce C. Breger, Gregory A. Ellis, Scott A. Walper, Kimihiro Susumu, and Igor L. Medintz
16. A Four-Enzyme Nanoassembly Consisting of Hydrolases and Oxidoreductases for Multi-Step Cascade Reactions
Archontoula Giannakopoulou, Michaela Patila, Nikolaos Chalmpes, Dimitrios Gournis, and Haralambos Stamatis
17. A Bi-Enzymatic Immobilized Nanobiocatalyst for The Biotransformation of Oleuropein to Hydroxytyrosol
Archontoula Giannakopoulou, Alexandra V. Chatzikonstantinou, Aliki Tsakni, Christos L. Chochos, Dimitra Houhoula, and Haralambos Stamatis
18. A Simple Recombinant E. coli Cell Lysate-Based Biocatalyst for ATP-Dependent Multi-Step Reactions
Natalia Bartzoka, Thomas D. Loan, Hideki Onagi, and Apostolos Alissandratos
19. CO2 to Methanol: A Highly Efficient Enzyme Cascade
Io Antonopoulou, Ulrika Rova, and Paul Christakopoulos
20. Multienzyme Catalysis in Phase-Separated Protein Condensates
Miao Liu, Xi Chen and Jiang Xia
21. Robust and Continuous Lipase-Catalyzed Reactions in Deep Eutectic Solvents: Low Viscosity and Double CLEA-Lentikats Immobilization (CLEA-LK-Lipases)
Nadia Guajardoa and Pablo Domínguez De María
22. Enzymatic Photometric Assays for the Selective Detection of Halides
Qingyun Tang, Askin S. Aslan-Üzel, Eva D. Schuiten, Christoffel P. S. Badenhorst, Ioannis V. Pavlidis, and Uwe T. Bornscheuer
