Sioud Ribozymes and siRNA protocols

2. Auflage 2004
ISBN: 978-1-59259-746-8
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 252, 624 Seiten, eBook

Reihe: Methods in Molecular Biology

Zielgruppe

Research

Autoren/Hrsg.

Sioud, Mouldy

Inhaltsverzeichnis

Ribozyme- and siRNA-Mediated mRNA Degradation.- Combination of Chemical and Enzymatic RNA Synthesis.- Determination of Kinetic Parameters for Hammerhead and Hairpin Ribozymes.- Real-Time Monitoring of RNA and DNA Reactions by Fluorescence Detection.- Quantification of Ribozyme Target RNA Using Real-Time PCR.- Analysis of Ribozyme Structure and Function by Nucleotide Analog Interference Mapping.- Analysis of Global Conformational Transitions in Ribozymes.- In Vivo Detection of Ribozyme Cleavage Products and RNA Structure by Use of Terminal Transferase-Dependent PCR.- Identification of Efficient Cleavage Sites in Long-Target RNAs.- Selection In Vitro of Allosteric Ribozymes.- Regulation of Ribozyme Cleavage Activity by Oligonucleotides.- Tetracycline-Regulated Expression of Hammerhead Ribozymes In Vivo.- Ribozyme Expression Systems.- Design and Expression of Chimeric U1/Ribozyme Transgenes.- Design and Validation of Therapeutic Hammerhead Ribozymes for Autosomal Dominant Diseases.- Helicase-Attached Novel Hybrid Ribozymes.- Functional Gene Discovery Using Hybrid Ribozyme Libraries.- Maxizyme Technology.- Target-Site Selection for the 10–23 DNAzyme.- In Vitro Selected RNA-Cleaving DNA Enzymes From Combinatorial Libraries.- Nucleic Acid Sequence Analysis Using DNAzymes.- Crystallization of the Hairpin Ribozyme.- An Experimental Method for Selecting Effective Target Sites and Designing Hairpin Ribozymes.- Design and Optimization of Sequence-Specific Hairpin Ribozymes.- Design, Targeting, and Initial Screening of sTRSV-Derived Hairpin Ribozymes for Optimum Helix 1 Length and Catalytic Efficiency In Vitro.- Optimization and Application of the Group I Ribozyme Trans-Splicing Reaction.- Repair of Myotonic Dystrophy Protein Kinase (DMPK) Transcripts by Trans-SplicingRibozymes.- General Design and Construction of RNase P Ribozymes for Gene-Targeting Applications.- In Vitro Selection of RNase P Ribozymes That Efficiently Cleave a Target mRNA.- In Vitro Selection of External Guide Sequences for Directing Human RNase P to Cleave a Target mRNA.- RNase P-Mediated Inhibition of Viral Growth by Exogenous Administration of Short Oligonucleotide External Guide Sequence.- RNase P Ribozyme As an Antiviral Agent Against Human Cytomegalovirus.- Inhibition of Gene Expression by Nucleic Acid Enzymes in Rodent Models of Human Disease.- Potential Design Rules and Enzymatic Synthesis of siRNAs.- RNA Interference (RNAi) With RNase III-Prepared siRNAs.- RNAi Expression Vectors in Mammalian Cells.- Gene-Array Analysis of Glioma Cells After Treatment With an Anti-PKC? siRNA.- RNAi in Living Mice.- Construction and Transfection of PCR Products Expressing siRNAs or shRNAs in Mammalian Cells.- Systemic Delivery of Synthetic siRNAs.- Adenovirus-Delivered siRNA.- RNAi Expression Vectors in Plant Cells.- Delivery Agents for Oligonucleotides.- Selection of Peptides for Specific Delivery of Oligonucleotides Into Cancer Cells.- Clinical Gene Therapy Research Utilizing Ribozymes.- Critical Steps in the Implementation of Hematopoietic Progenitor-Cell Gene Therapy Using Ribozyme Vectors.