Antibody, Biosensor, and Nucleic Acid Technologies
Buch, Englisch, 512 Seiten, Format (B × H): 155 mm x 235 mm, Gewicht: 838 g
ISBN: 978-94-011-6978-3
Verlag: Springer Netherlands
The past decade has seen the growth of analytic methodologies based on antibody and DNA probe technologies. In fact, these two technologies have revolutionized the whole field of human, veterinary, and agriculture diagnostics. The emphasis has been to develop tests that are fast, reliable, and user-friendly, and that can be performed on site by attending individuals or patients. The tests should also be amenable to automation for high throughput for use in commercial and hospital laboratories. The mushroomed growth of such diagnostics has created a niche for a book that can bring together the perspective of the upcoming technologies that are otherwise in their infancy. The intent of this book is to present readers with these state-of-the-art futuristic methods, which will be the hallmark of diagnostics in the twenty-first century. These new technologies have lain the foundation for a plethora of new diagnostic companies whose products are still not fully accepted because of practical challenges and the newness of their ideas. The chapters in this book have been written by world-renowned authorities in their fields of expertise. All aspects of diagnostics are covered: clinical, agricul ture, veterinary, and environmental. State-of-the-art immunodiagnostic methods such as nephelometric immunoassay, time-resolved fluoroimmunoassay, and threshold immunoassay are discussed. Chapters on DNA-probe-based diagnostics and PCR technology are also included, since they are finding their ways rapidly because of their specificity and ease of probe production. A chapter on biosensors discusses the latest development on this topic and its possible potential in modem-day diagnostics.
Zielgruppe
Research
Autoren/Hrsg.
Weitere Infos & Material
1. Diagnostics—Where We Were, Where We Are, and Where We are Going: An Introduction.- Immunoassay Principle.- Application of Diagnostics.- Agriculture and Environmental Diagnostics.- Biosensors.- Nucleic-Acid-Based Diagnostics.- Automation in Diagnostics and Review.- Acknowledgments.- I. Principles and Methods.- 2. Homogeneous Enzyme Immunoassays: EMIT®.- 3. CEDIA®: A Recombinant-Based Homogeneous Enzyme Immunoassay.- 4. Fluorescence Polarization Immunoassay and Therapeutic Drug Monitoring and Toxicology.- 5. Time-Resolved Fluoroimmunoassays.- 6. Chemiluminescence Enzyme Immunoassay and Its Use in Diagnostics.- 7. Enzyme Immunoassay System Enhanced by Bioluminescence.- 8. Spin Immunoassay and Its Use in Diagnostics.- 9. Nephelometric Immunoassay for Therapeutic Drug Monitoring.- II. Applications of Diagnostics.- 10. Instrument-Free Quantitative Test Systems.- 11. The Eclipse ICA®: An Immunochemical and Clinical Chemistry Assay System for Near-Patient Testing.- 12. Immunoassays for Tropical Diseases.- 13. Connective Tissue Diseases Diagnostic Approach.- 14. Diagnostics of Trace Elements and Their Role in Senile Cataract in Humans.- III. Agriculture and Environmental Diagnostics.- 15. Agriculture, Veterinary, and Environmental Diagnostics.- 16. Diagnostics in Agriculture.- 17. Immunoassays for Pesticides, Aflatoxins, Polychlorinated Dibenzo-p-dioxins and Plant Pathogens.- 18. Immunodetection of Fungi and Their Products.- IV. Biosensors.- 19. Biosensor Principles and Applications.- 20. Immunosensors: Their Future Role in Clinical Diagnostics.- V. Nucleic Acids-Based Diagnostics and Their Application.- 21. DNA Probe-Based Diagnostics.- 22. Polymerase Chain Reaction and Its Use in Diagnostics.- 23. AIDS Diagnostics.- VI. Automation in Diagnostics.- 24. Robotics andAutomation in Diagnostics.- VII. Review of Diagnostics and Future Directions.- 25. Diagnostics: Immunoassays, Nucleic Acid Probes, and Biosensors—Two Decades of Development, Current Status, and Future Projections in Clinical, Environmental, and Agricultural Applications.
