Buch, Englisch, 308 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 809 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, 308 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 809 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-3592-6
Verlag: Springer
This volume details multiple areas of new and emerging methods to develop the next generation of immunotherapy treatments. Chapters guide readers through analysis and characterisation of the interactions between tumour and immune cells, and cell engineering tools for cancer treatment, to provide a unique and compelling set of techniques instrumental to work with, and engineer, immune cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Cancer Immunotherapy: Methods and Protocols aims to ensure successful results in the further study of this vital field.
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Weitere Infos & Material
1. Isolation of live immune cells from the tumour microenvironment by FACS
Aikaterini Kafka, Christos Ermogenous, and Luigi Ombrato
2. In vitro evaluation of cancer cell immunogenicity and antigen-specific T-cell cytotoxicity by flow cytometry
Martina Musella, Nicoletta Manduca, Ester Maccafeo, Eliana Ruggiero, and Antonella Sistigu3. Retroviral transduction of human primary T cells followed by real-time T-cell mediated cancer cell cytolysis analysis
Anne Rahbec, Reno Debets, Per thor Stratenand Marlies J.W. Peeters
4. Expansion and retroviral transduction of primary murine T cells for CAR T cell therapy
Pauline Loos, Lauralie Short, Gillian Savage, and Laura Evgin
5. In Situ Decellularisation of Tissues applied to the topographical analysis of tumour associated extracellular matrix
Alejandro E Mayorca Guiliani
6. Monitoring cell cytoskeleton variations upon piezoelectric stimulation: Implications for the immune system
Özlem Sen, Carlotta Pucci, and Gianni Ciofani7. Preparation method and in vitro characterization of nanoparticles sensitive to tumor microenvironment
Naym Blal and Daniela Guarnieri
8. A new microfluidic device to facilitate functional precision medicine assays
Albert Manzano-Muñoz, Jose Yeste, María A. Ortega, Josep Samitier, Javier Ramón-Azcón, and Joan Montero
9. Kinetic detection of apoptosis events via caspase 3/7 activation in a tumor-immune microenvironment on a chip
Bertani F.R., Dabbagh Moghaddam F., Panella C., Giannitelli S.M., Peluzzi V., Gerardino A., Rainer A., Roscilli G., De Ninno A. and Businaro L.
10. Hypoxic 3D Tumor Model for Evaluating of CAR-T Cell Therapy In Vitro
Jeong Min Oh and Keyue Shen
11. Rapid screening of CAR T cell functional improvement strategies by highly-multiplexed single-cell secretomics
Dragana Slavkovic-Lukic, Jessica Fioravanti, Azucena Martín-Santos, Edward Han, Jing Zhou, and Luca Gattinoni
12. Genome editing in CAR-T cells using CRISPR/Cas9 technology
Irene Andreu-Saumell, Alba Rodriguez-Garcia, and Sonia Guedan
13. Genetic modification of tumor-infiltrating lymphocytes, peripheral T cells, and T cell model cell lines
Hadas Weinstein-Marom, Dayana Blokon-Kogan, Maya Levi-Mann, Chaja Katzman, Shira Shalev, Masha Zaitsev, Michal J. Besser, Ronnie Shapira-Frommer, Gideon Gross, Orit Itzhaki, and Lior Nissim
14. Transposon-based manufacturing of human CAR-T cells
Megan Tennant and Richard O’Neil
15. Redirecting human conventional and regulatory T cells using chimeric antigen receptors
Capers M. Zimmerman, Rob A. Robino, Russell W. Cochrane, Matthew D. Dominguez, and Leonardo M.R. Ferreira
16. How to Test Human CAR T cells in Solid Tumors, The Next Frontier of CAR T Cell Therapy
Russell W. Cochrane,Andrew Fiorentino, Eva Allen, Rob A. Robino, Jaime Quiroga, and Leonardo M.R. Ferreira
17. Nano-Optogenetic CAR-T Cell Immunotherapy
Nhung Thi Nguyen, Siyao Liu, Gang Han, Yubin Zhou, and Kai Huang
18. A non-viral piggyBac transposon-mediated method to generate large-scale CAR-NK cells from human peripheral blood primary NK cells
Zhicheng Du, Tianzhi Zhao, Xianjin Chen, Shijun Zha and Shu Wang
19. Engineering probiotic E.coli Nissle 1917 for release of therapeutic nanobodies
Candice Gurbatri and Tal Danino
