Gene Expression Profiling

1. Auflage. Softcover version of original hardcover Auflage 2004
ISBN: 978-1-61737-429-6
Verlag: Humana Press

Buch, Englisch, Band 258, 168 Seiten, Previously published in hardcover, Format (B × H): 155 mm x 235 mm, Gewicht: 316 g

Reihe: Methods in Molecular Biology

Leading scientists in gene expression methodology and bioinformatics data analysis describe readily reproducible methods for measuring RNA levels in cells and tissues. The techniques presented include new methods for applying the Affymetrix GeneChip®, SAR-SAGE, StaRT-PCR, SSH, the Invader Assay®, and ADGEM. The authors also provide critical bioinformatics insight and resources for data analysis and management. By distilling the basic underlying principles of many methods to a few straightforward concepts, investigators can easily choose the method most appropriate to their application.

Zielgruppe

Research

Autoren/Hrsg.

Shimkets, Richard A.

Fachgebiete

• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Biowissenschaften Genetik und Genomik (nichtmedizinisch)

Inhaltsverzeichnis

Technical Considerations in Quantitating Gene Expression.- Gene Expression Quantitation Technology Summary.- Standardized RT-PCR and the Standardized Expression Measurement Center.- GeneCalling.- Invader Assay for RNA Quantitation.- Monitoring Eukaryotic Gene Expression Using Oligonucleotide Microarrays.- Amplified Differential Gene Expression Microarray.- Suppression Subtractive Hybridization.- Small Amplified RNA-SAGE.- Gene Expression Informatics.