Shimkets Gene Expression Profiling

2004
ISBN: 978-1-59259-751-2
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 258, 168 Seiten, eBook

Reihe: Methods in Molecular Biology

Leading scientists in gene expression methodology and bioinformatics data analysis describe readily reproducible methods for measuring RNA levels in cells and tissues. The techniques presented include new methods for applying the Affymetrix GeneChip®, SAR-SAGE, StaRT-PCR, SSH, the Invader Assay®, and ADGEM. The authors also provide critical bioinformatics insight and resources for data analysis and management. By distilling the basic underlying principles of many methods to a few straightforward concepts, investigators can easily choose the method most appropriate to their application.

Zielgruppe

Research

Autoren/Hrsg.

Shimkets, Richard A.

Inhaltsverzeichnis

Technical Considerations in Quantitating Gene Expression Richard A. Shimkets Gene Expression Quantitation Technology Summary Richard A. Shimkets Standardized RT-PCR and the Standardized Expression Measurement Center James C. Willey, Erin L. Crawford, Charles R. Knight, K. A. Warner, Cheryl A. Motten, Elizabeth A. Herness, Robert J. Zahorchak, and Timothy G. Graves GeneCalling: Transcript Profiling Coupled to a Gene Database Query Richard A. Shimkets Invader Assay for RNA Quantitation Marilyn C. Olson, Tsetska Takova, LuAnne Chehak, Michelle L. Curtis, Sarah M. Olson, and Robert W. Kwiatkowski Monitoring Eukaryotic Gene Expression Using Oligonucleotide Microarrays Jennifer Lescallett, Marina E. Chicurel, Robert Lipshutz, and Dennise D. Dalma-Weiszhausz Amplified Differential Gene Expression Microarray, Zhijian J. Chen and Kenneth D. Tew Suppression Subtractive Hybridization Denis V. Rebrikov, Sejal M. Desai, Paul D. Siebart, and Sergey A. Lukyanov Small Amplified RNA-SAGE Catheline Vilain and Gilbert Vassart Gene Expression Informatics Martin D. Leach Index