E-Book, Englisch, 270 Seiten, E-Book
Serre Diagnostic Techniques in Genetics
1. Auflage 2007
ISBN: 978-0-470-87026-6
Verlag: John Wiley & Sons
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)
E-Book, Englisch, 270 Seiten, E-Book
ISBN: 978-0-470-87026-6
Verlag: John Wiley & Sons
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)
Recent developments within molecular biology and geneticengineering have led to huge advances and changes within thebiological sciences especially within the field of human genetics.Diagnostic Techniques in Genetics offers an importantoverview of how DNA or RNA technology may be applied to a large setof genetic diagnoses.
The first part of the book focuses on DNA/RNA applications andincludes many of the latest developments in the field combined withroutine procedures of genetic diagnoses, for example cloning andsequencing DNA. The DNA applications presented in the first chapterare then each applied to a specific kind of genetic diagnosis andthe text concludes with a chapter devoted to populationgenetics.
First published in French by Dunod in 2002, this book is anexcellent reference for students taking courses in molecularbiology, medicine and medical genetics. It is also a usefulintroduction for postgraduate students and researchers in the fieldwho require a general overview of genetic diagnoses.
Weitere Infos & Material
Preface.
List of Contributors.
1. Techniques and Tools in Molecular Biology Used in GeneticDiagnoses.
1.1 Nucleic acids.
1.2 The different types of genetic material studied.
1.3 The enzymatic tools for in vitro treatment ofDNA.
1.4 DNA fragmentation and study of the fragments.
1.5 Selective amplification of a nucleotide sequence.
1.6 DNA fragment ligation: recombinant DNA and cloning.
1.7 DNA fragment sequencing.
1.8 Modification of the sequence of a DNA fragment:site-directed mutagenesis.
1.9 Molecular hybridization techniques and applications.
1.10 Other techniques to study allelic diversity.
2. The Diagnosis of Inherited Diseases.
2.1 Introduction.
2.2 Example diagnoses for autosomal diseases.
2.3 Example diagnoses for X-linked diseases.
2.4 Neurodegenerative diseases.
2.5 References and Bibliography.
3. Molecular Diagnosis in Oncology.
3.1 General introduction.
3.2 Cellular pathways targeted by the tumour process.
3.3 Types of genetic alteration leading to cancer.
3.4 Alteration origins: the role of the repair genes.
3.5 Benefits of molecular studies to patient healthcare.
3.6 Genetic predisposition to cancers.
3.7 Genetic tests for cancer predisposition.
3.8 Conclusions and perspectives.
3.9 References.
4. Applications of Molecular Biology to Cytogenetics.
4.1 Introduction.
4.2 Molecular diagnosis of anomalies in the number ofchromosomes.
4.3 Chromosomal microdeletions.
4.4 Uniparental disomies.
4.5 Conclusions and perspectives.
4.6 References.
5. Screening and Identification of Pathogenic and ExogenicAgents.
5.1 Clinical virology.
5.2 Clinical bacteriology.
5.3 Detection of GMOs.
5.4 References and Bibliography.
6. Identification Using Genetic Fingerprints.
6.1 Introduction.
6.2 Genetic fingerprints by the analysis of nuclear DNA.
6.2.4 A special case: the Y chromosome sequence.
6.3 Genetic fingerprints with mitochondrial DNA.
6.4 Society facing the question of genetic fingerprintfiles.
6.5 Conclusions.
6.6 Bibliography.
7. Molecular Genetics and Populations.
7.1 Hardy-Weinberg equilibrium and measures of geneticdiversity.
7.4 Diagnosis with genetic testing: cystic fibrosis - anacademic case.
7.5 Gametic disequilibrium.
7.6 Reference and Bibliography.
Index.
