Scopes | Protein Purification | E-Book | sack.de
E-Book

E-Book, Englisch, 329 Seiten, eBook

Reihe: Springer Advanced Texts in Chemistry

Scopes Protein Purification

Principles and Practice
2. Auflage 1987
ISBN: 978-1-4757-1957-4
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

Principles and Practice

E-Book, Englisch, 329 Seiten, eBook

Reihe: Springer Advanced Texts in Chemistry

ISBN: 978-1-4757-1957-4
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark



New textbooks at alllevels of chemistry appear with great regularity. Some fields like basic biochemistry, organic reaction mechanisms, and chemical thermodynamics are weil represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a reallack ofup-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research which is advancing the field. lt is not often easy to persuade such individuals to settime aside to help spread the knowledge they have accumu lated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instructive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In some cases the availability of texts in active research areas should help stimulate the creation of new courses. NewYork CHARLES R. CANTOR Preface to the Second Edition The original plan for the first edition of this book was to title it Enzyme Purification: Princip/es and Practice.

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1 The Enzyme Purification Laboratory.- 2 Making an Extract.- 3 Separation by Precipitation.- 4 Separation by Adsorption. I. General Principles.- 5 Separation by Adsorption. II. Specific Adsorbents.- 6 Separation in Solution.- 7 Optimization of Procedures; Recombinant Protein Techniques.- 8 Maintenance of Active Enzymes.- 9 Measurement of Enzyme Activity.- 10 Analysis for Purity: Crystallization.- Appendix A.- Addition of Precipitants.- Appendix B.- Solutions for Measuring Protein Concentration.- References.



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