Scopes Protein Purification

Principles and Practice
Erscheinungsjahr 2013
ISBN: 978-1-4757-1770-9
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 282 Seiten, eBook

Reihe: Springer Advanced Texts in Chemistry

New textbooks at all levels of chemistry appear with great regularity. Some fields like basic biochemistry, organic reaction mechanisms, and chemical ther modynamics are well represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the grad uate level, suffer from a real lack of up-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research which is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instructive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In some cases the availability of texts in active research areas should help stimulate the creation of new courses. New York CHARLES R.

Zielgruppe

Research

Autoren/Hrsg.

Scopes, R.K.

Inhaltsverzeichnis

1 The Enzyme Purification Laboratory.- 2 Making an Extract.- 3 Separation by Precipitation.- 4 Separation by Adsorption.- 5 Separation in Solution.- 6 Maintenance of Active Enzymes.- 7 Optimization of Procedures and Following a Recipe.- 8 Measurement of Enzyme Activity.- 9 Analysis for Purity; Crystallization.- Appendix A.- Appendix B.- Solutions for Measuring Protein Concentration.- References.