Satir / Christensen Cilia
1. Auflage 2016
ISBN: 978-1-4939-3789-9
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark
Methods and Protocols
E-Book, Englisch, 259 Seiten
Reihe: Springer Protocols
ISBN: 978-1-4939-3789-9
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark
This volume is the result of an explosion of
molecular-based research on Cilia, which began with the discovery of the
universality of intraflagellar transport (IFT) and ciliary genomics/proteomics.
The chapters in this book cover topics such as: high resolution imaging and
functional characterization of sensory and primary cilia in mammalian cells and
zebrafish, methods to study ciliary-mediated chemoresponse in
Paramecium
,
and methods to study centrosomes and cilia in
C. elegans
and
Drosophila
.
Written in the highly successful Methods in Molecular Biology series format,
chapters include introduction to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and cutting-edge,
Cilia:
Methods and Protocols
is broad and covers motile, sensory, and primary
cells. It is a valuable resource to anyone interested in entering the field of
ciliary biology using model organisms, including flagellate algae, ciliates,
planaria, nematodes, insects, zebrafish, and mammalian cells.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Weitere Infos & Material
Methods for Studying Ciliary Import Mechanisms.- Targeting of ASH Domain-Containing Proteins to the Centrosome.- Morphological and Functional Characterization of the Ciliary Pocket by Electron and Fluorescence Microscopy.- Methods to Study Interactions between Ciliogenesis and Autophagy.- Recombinant Reconstitution and Purification of the IFT B Core Complex from Chlamydomonas reinhardtii.- Methods for Studying Movement of Molecules within Cilia.- A FRAP-Based Method for Monitoring Molecular Transport in Ciliary Photoreceptor Cells In Vivo.- Kymographic Analysis of Transport in an Individual Neuronal Sensory Cilium in Caenorhabditis elegans.- Visualization and Manipulation of Cilia and Intraciliary Calcium in the Zebrafish Left-Right Organizer.- Methods for Studying Ciliary-Mediated Chemoresponse in Paramecium.- STED and STORM Superresolution Imaging of Primary Cilia.- CLEM Methods forStudying Primary Cilia.- Methods for Visualization of Neuronal Cilia.- Methods to Study Centrosomes and Cilia in Drosophila.- Analysis of Axonemal Assembly during Ciliary Regeneration in Chlamydomonas.- Planaria as a Model System for the Analysis of Ciliary Assembly and Motility.
