Sarker Natural Products Isolation

2. Auflage 2005
ISBN: 978-1-59259-955-4
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 20, 516 Seiten, eBook

Reihe: Methods in Biotechnology

The term “natural products” spans an extremely large and diverse range of chemical compounds derived and isolated from biological sources. Our interest in natural products can be traced back thousands of years for their usefulness to humankind, and this continues to the present day. Compounds and extracts derived from the biosphere have found uses in medicine, agriculture, cosmetics, and food in ancient and modern societies around the world. Therefore, the ability to access natural products, understand their usefulness, and derive applications has been a major driving force in the field of natural product research. The first edition of Natural Products Isolation provided readers for the first time with some practical guidance in the process of extraction and isolation of natural products and was the result of Richard Cannell’s unique vision and tireless efforts. Unfortunately, Richard Cannell died in 1999 soon after completing the first edition. We are indebted to him and hope this new edition pays adequate tribute to his excellent work. The first edition laid down the “ground rules” and established the techniques available at the time. Since its publication in 1998, there have been significant developments in some areas in natural product isolation. To capture these developments, publication of a second edition is long overdue, and we believe it brings the work up to date while still covering many basic techniques known to save time and effort, and capable of results equivalent to those from more recent and expensive techniques.

Zielgruppe

Research

Autoren/Hrsg.

Sarker, Satya D.

Inhaltsverzeichnis

Natural Product Isolation.- Initial and Bulk Extraction.- Supercritical Fluid Extraction.- An Introduction to Planar Chromatography.- Isolation of Natural Products by Low-Pressure Column Chromatography.- Isolation by Ion-Exchange Methods.- Separation by High-Speed Countercurrent Chromatography.- Isolation by Preparative High-Performance Liquid Chromatography.- Hyphenated Techniques.- Purification by Solvent Extraction Using Partition Coefficient.- Crystallization in Final Stages of Purification.- Dereplication and Partial Identification of Compounds.- Extraction of Plant Secondary Metabolites.- Isolation of Marine Natural Products.- Isolation of Microbial Natural Products.- Purification of Water-Soluble Natural Products.- Scale-Up of Natural Product Isolation.- Follow-Up of Natural Product Isolation.