Buch, Englisch, Band 2227, 267 Seiten, Format (B × H): 178 mm x 254 mm, Gewicht: 532 g
Reihe: Methods in Molecular Biology
Innovative Diagnostic and Research Protocols
Buch, Englisch, Band 2227, 267 Seiten, Format (B × H): 178 mm x 254 mm, Gewicht: 532 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-1018-3
Verlag: Springer US
This volume describes a range of methods used in complement laboratories and how to interpret the data. Chapters detail methods for depletion of IgG and IgM, quantification of complement proteins, C3dg quantification, complement C3 deposition on endothelial cells, anti-C1q auto-antibodies, and methods for assessment of interactions of proteins with heme. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and clearly written, The Complement System: Innovative Diagnostic and Research Protocols aims to ensure successful results in the further study of this vital field.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
1. The Benefits of Complement Measurements for the Clinical Practice
Anne Grunenwald and Lubka T. Roumenina
2. Method for Depletion of IgG and IgM from Human Serum as Naive Complement Source
Seline A. Zwarthoff, Simone Magnoni, Piet C. Aerts, Kok P. M. van Kessel, and Suzan H.M. Rooijakkers
3. Quantification of Complement Proteins with Special Reference to C1q: Multiplex vs ELISA vs Rocket Immunoelectrophoresis vs Nephelometry
Kerstin Sandholm, Barbro Persson, Sliva Abdalla, Camilla Mohlin, Bo Nilsson, and Kristina N Ekdahl
4. C3dg Quantification by PEG-precipitation and ELISA or TRIFMA
Anne Troldborgand Jens Christian Jensenius
5. Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope Based Enzyme-Linked Immunosorbent Assay
Per H. Nilsson, Kristin Pettersen, Martin Oppermann, Espen Skjeflo, Hilde Fure, Dorte Christiansen, and Tom Eirik Mollnes
6. Sheep Erythrocytes Preparation for Hemolytic Tests Exploring Complement Functional Activities
Melchior Chabannes, Pauline Bordereau, Paula Vieira Martins, and Marie-Agnès Dragon-Durey
7. Hemolytic Tests Exploring Factor H Functional Activities
Melchior Chabannes, Shambhuprasad K Togarsimalemath, and Marie-Agnès Dragon-Durey
8. Functional Hemolytic Test for Complement Alternative Pathway Convertase Activity
Marloes A.H.M. Michels, Nicole C.A.J. van de Kar, Elena B. Volokhin, and Bert (L) P.W.J. van den Heuvel
9. Complement C3 Deposition on Endothelial Cells Revealed by Flow Cytometry
Idris Boudhabhay, Anne Grunenwald, and Lubka T. Roumenina
10. Anti-C1q Auto-Antibodies: Standard Quantification and Innovative ELISA
Kinga Csorba, Lucias Schirmbeck, Denise Dubler, and Marten Trendelenburg
11. Anti-C1-Inhibitor Auto-Antibodies Detection by ELISA
Chiara Suffritti, Sonia Caccia, Silvia Berra, Debora Parolin, and Marco Cicardi
12. Anti-ficolin-2 and anti-ficolin-3 Autoantibodies Detection by ELISA
Chantal Dumestre-Pérard and Nicole M. Thielens13. Detection of anti-C3b Autoantibodies by ELISA
Maria Radanova, Lubka Roumenina, and Vasil Vasilev14. Detection of Complement Factor B Autoantibodies by ELISA
Mihály Józsi and Barbara Uzonyi
15. Detection of C3 Nephritic Factor by Hemolytic Assay
Melchior Chabannes, Véronique Frémeaux-Bacchi, Sophie Chauvet
16. Detection of Genetic Rearrangements inthe Regulators of Complement Activation RCA Cluster by High Throughput Sequencing and MLPA
Jesús García-Fernández, Susana Vilches-Arroyo, Leticia Olavarrieta, Julián Pérez-Pérez, and Santiago Rodríguez de Córdoba
17. Complement Detection in Mouse Kidneys by Immunofluorescence
Jennifer Laskowski and Joshua M. Thurman
18. Complement Detection in Human Tumors by Immunohistochemistry and Immunofluorescence
Marie V. Daugan, Margot Revel, Laetitia Lacroix, Catherine Sautès-Fridman, Wolf H. Fridman, and Lubka T. Roumenina
19. Analysis Of The Ligand Recognition Specificities Of Human Ficolins Using Surface Plasmon Resonance
Nicole M. Thielens, Evelyne Gout, Monique Lacroix, Jean-Baptiste Reiser, and Christine Gaboriaud
20. Methods for Assessment of Interactions of Proteins with Heme - Application for Complement Proteins and Immunoglobulins
Margot Reveland Jordan D. Dimitrov
21. Evaluation of Binding Kinetics and Thermodynamics of Antibody-antigen Interactions and Interactions Involving Complement Proteins
Sofia Rossiniand Jordan D. Dimitrov
22. Purification of Human Complement Component C4 and Sample Preparation for Structural Biology Applications
Alessandra Zarantonello, Sofia Mortensen, Nick S. Laursen, and Gregers R. Andersen
