Rothe Electrophoresis of Enzymes

Laboratory Methods
Erscheinungsjahr 2012
ISBN: 978-3-642-79069-0
Verlag: Springer
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 307 Seiten, eBook

Reihe: Springer Lab Manuals

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Rothe, Gunter M.

Inhaltsverzeichnis

1 Introduction.- 1.1 Enzyme Classification.- 1.2 Enzyme Subunit Composition.- 1.3 Isozyme Classification.- 1.4 References.- 2 Extraction of Enzymes from Tissues, Cells and Cell-Organelles.- 2.1 Methods to Extract Enzymes from Microorganisms.- 2.2 Methods to Extract Enzymes from Animal Soft Tissue.- 2.3 Differential Extraction of Cytosolic and Mitochondrial Enzymes from Animal Soft Tissue.- 2.4 Extraction of Insects.- 2.5 Extraction of Plant Tissues.- 2.6 Concentration of Diluted Enzyme Extracts.- 2.7 Mammalian Blood.- 2.8 Pancreatic Islets.- 2.9 Isolation of Subcellular Organelles.- 2.10 Catalytic Activities of Enzymes Forming a Common Metabolic Sequence.- 2.11 References.- 3 Methods for Separating Native Enzymes.- 3.1 General Considerations.- 3.2 Cellulose Acetate Electrophoresis.- 3.3 Starch Gel Electrophoresis.- 3.4 References.- 3.5 Analytical Polyacrylamide Gel Electrophoresis for Separating Native Enzymes.- 3.6 2-D-Electrophoresis under Non-Denaturing Conditions.- 3.7 References.- 4 Sodium Dodecylsulphate Electrophoresis.- 4.1 Homogeneous Buffer Systems.- 4.2 Discontinuous Buffer Systems.- 4.3 Gradient Gel Systems.- 4.4 Enzyme Visualization Following SDS-Electrophoresis.- 4.5 References.- 5 Chemistry of Enzyme Visualization.- 5.1 Methods for Visualizing Oxidative Enzymes.- 5.2 Methods for Visualizing Transferases.- 5.3 Methods for Visualizing Hydrolases.- 5.4 Methods for Visualizing Lyases.- 5.5 Methods for Localizing Isomerases and Ligases.- 5.6 Visualization of Enzymes by Different Methods.- 5.7 References.- 6 A Compilation of Protocols to Visualize Enzymes.- 6.1 Index to Enzyme Recipes and Separation Systems.- 6.2 Protocols to Visualize Enzymes Following Electrophoretic Separation.- 6.3 References.- 7 Data Evaluation in Population Genetics and Evolution.- 7.1 Allozymes as Gene Markers.- 7.2 Population Genetic Measures.- 7.3 Calculation of Dendrograms.- 7.4 References.