Rizzuto / Fasolato Imaging Living Cells

Erscheinungsjahr 2012
ISBN: 978-3-642-60003-6
Verlag: Springer
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 394 Seiten, Web PDF

Reihe: Springer Lab Manuals

Improved technology for imaging living cells, specific cellular targets and organelles is having a dramatic impact on basic and applied research. By combining optical design and molecular genetics, a new series of tools is being developed and successfully applied together with classical probes. Novel labelling strategies, better software for image enhancement and analysis are now available and allow image acquisition with greater speed and precision. This lab manual, intended as for bench-top use, is suitable for both scientists and graduate students, combines an update on the most advanced imaging procedures with detailed protocols. Examples, cleverly selected from the wide repertoire of cell pyhsiology, cover different functional aspects such as distribution of multiple ions, electrical activity, exo-endocytosis, gene expression, and the cell cycle.

Zielgruppe

Research

Autoren/Hrsg.

Rizzuto, Rosario

Fasolato, Cristina

Inhaltsverzeichnis

The Instruments.- Section 1 Strategies for Quantitative Digital Imaging of Biological Activity in Living Cells with Ion-Sensitive Fluorescent Probes.- Section 2 High Resolution 3-D Imaging of Living Cells by Image Restoration.- Fluorescent Dyes.- Section 3 Imaging Calcium in the Cytoplasm and in Organelles with Fluorescent Dyes: General Principles.- Section 4 Confocal Calcium Imaging.- Section 5 Imaging Whole Organs — Single cell [Ca2+]i measurements in the perfused liver.- Section 6 Simultaneous Measurement of Membrane Current and Intracellular Calcium.- Section 7 Quantitative Calcium Imaging in Brain Slices.- Section 8 Imaging of Cytosolic Sodium and pH Using SBFI and BCECF.- Section 9 Common Methods for Measuring Apoptotic Cell Death by Flow Cytometry.- Section 10 Monitoring Synaptic Exocytosis and Endocytosis by Optical Techniques.- Recombinant Probes.- Section 11 Targeting, Expressing and Calibrating Recombinant Aequorin.- Section 12 Imaging Ca2+ in Small Mammalian Cells with Recombinant Targeted Aequorin.- Section 13 Analysis of Regulated Gene Expression by Microinjection and Digital Luminescence Imaging of Single Living Cells.- Section 14 Imaging Green Fluorescent Proteins in Mammalian Cells.- Section 15 The New Green Fluorescent Protein Mutants and their Applications.- Section 16 Imaging Green Fluorescent Protein in Transgenic Plants.