Trinucleotide Repeats

Methods and Protocols
1. Auflage 2020
ISBN: 978-1-4939-9783-1
Verlag: Springer

Buch, Englisch, 286 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 765 g

Reihe: Methods in Molecular Biology

This detailed book contains techniques to explore the unusual properties of the peculiar microsatellites known as trinucleotide repeats.  Beginning with technical challenges raised by secondary structure-forming repeats and their propensity to contract and expand over time, the book continues with genetic screens in Saccharomyces cerevisiae , experimental systems to study trinucleotide repeat instability in human cells, dedicated experimental systems in appropriate cell types, as well as gene therapy approaches using the CRISPR-Cas family of endonucleases.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Trinucleotide Repeats: Methods and Protocols serves as avaluable aid to experts and newcomers alike who seek to investigate this fascinating and ever-expanding field of study.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Richard, Guy-Franck

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Humangenetik

Inhaltsverzeichnis

Experimenting with Trinucleotide Repeats: Facts and Technical Issues.- Fast Assays to Detect Interruptions in CTG.CAG Repeat Expansions.- Tracking Expansions of Stable and Threshold Length Trinucleotide Repeat Tracts In Vivo and In Vitro Using Saccharomyces cerevisiae .- Quantifying Replication Fork Progression at CTG Repeats by 2D Gel Electrophoresis.- Genetic Assays to Study Repeat Fragility in Saccharomyces cerevisiae .- Genetic Screens to Study GAA/TTC and Inverted Repeat Instability in Saccharomyces cerevisiae .- Monitoring Double-Strand Break Repair of Trinucleotide Repeats Using a Yeast Fluorescent Reporter Assay.- Analysis of Trinucleotide Repeat Stability by Integration at a Chromosomal Ectopic Site.- Experimental System to Study Instability of (CGG) Repeats in Cultured Mammalian Cells.- Assessing Triplet Repeat Expansions in Human SVG-A Cell Culture.- The Isolation and Analysis of the CGG-Repeat Size in Male and Female Gametes from a Fragile X Mouse Model.- In Vitro Synthesis and RNA Structure Probing of CUG Triplet Repeat RNA.- FISH Protocol for Myotonic Dystrophy Type 1 Cells.- Real Time Videomicroscopy and Semi-Automated Analysis of Brain Cell Culture Models of Trinucleotide Repeat Expansion Diseases.- Primary Cultures of Pure Embryonic Dorsal Root Ganglia Sensory Neurons as a New Cellular Model for Friedreich’s Ataxia.- GFP Reporters to Monitor Instability and Expression of Expanded CAG/CTG Repeats.- Gene Therapy for Huntington's Disease Using Targeted Endonucleases.