Acinetobacter baumannii

Methods and Protocols
1. Auflage 2019
ISBN: 978-1-4939-9117-4
Verlag: Springer

Buch, Englisch, Band 1946, 338 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 860 g

Reihe: Methods in Molecular Biology

This detailed volume serves clinicians and basic science researchers studying the increasingly antibiotic resistant Gram-negative bacterium Acinetobacter baumannii. Chapters detail microbiological techniques, biochemical techniques, clinical samples, and next generation omics techniques to characterize the organism at the molecular level.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and cutting-edge, Acinetobacter baumannii: Methods and Protocols aims to ensure successful results in the further study of this high priority area of antibiotic study.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Rather, Philip N.

Biswas, Indranil

Fachgebiete

• Naturwissenschaften Biowissenschaften Bakteriologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Infektionskrankheiten

Inhaltsverzeichnis

Specimen Collection, Processing, Culture, and Biochemical Identification of Acinetobacter spp..- Genotyping of Acinetobacter baumannii in Nosocomial Outbreak and Surveillance.- Antimicrobial Susceptibility Testing Methods for Acinetobacter spp.- Methods to Evaluate Colistin Heteroresistance in Acinetobacter baumannii.- Testing Metal Sensitivity of A. baumannii Strains: Survival in Copper Supplemented Liquid Media and on Copper-Containing Surfaces.- A New Method for Determination of Minimum Biofilm Eradication Concentration for Accurate Antimicrobial Therapy.- Transformation of Acinetobacter baumannii: Electroporation.- Methods for Natural Transformation in Acinetobacter baumannii.- Vesicle Mediated Gene Transfer in A. baumannii.- Targeted Gene Replacement in Acinetobacter baumannii.- RecET-Mediated Recombineering in Acinetobacter baumannii.- Methods for Tn-seq Analysis in Acinetobacter baumannii.- Tn7-Based Single Copy Insertion Vectors for Acinetobacter baumannii.- Distinguishing Colony Opacity Variants and Measuring Opacity Variation in Acinetobacter baumannii.- A Simple Static Biofilm Assay for Acinetobacter baumannii.- BioFluxTM 200 Microfluidic System to Study A. baumannii Biofilm Formation in a Dynamic Mode of Growth.- In Vitro Motility Assays for Acinetobacter Species.- Desiccation Tolerance Assays for Acinetobacter baumannii.- Measuring Intracellular Metal Concentration via ICP-MS Following Copper Exposure.- PBP Isolation and DD-Carboxypeptidase Assay.- Extraction and Visualization of Capsular Polysaccharide from Acinetobacter baumannii.- Isolation of Lipid Cell Envelope Components from Acinetobacter baumannii.- Methods for Detecting N-acyl-homoserine Lactone Production in Acinetobacter baumannii.- Isolation and Characterization of the Acinetobactin and Baumannoferrin Siderophores Produced by Acinetobacter baumannii.- Skin and Soft Tissue Models for Acinetobacter baumannii Infection.- Assessing Acinetobacter baumannii Virulence and Persistence in a Murine Model of Lung Infection.- Computational Prediction of sRNA in Acinetobacter baumannii.- Global Metabolic Analyses of Acinetobacter baumannii.- Reverse Vaccinology Approach to Potential Vaccine Candidates against Acinetobacter baumannii.