NMR-Based Metabolomics

Methods and Protocols
1. Auflage 2019
ISBN: 978-1-4939-9689-6
Verlag: Springer

Buch, Englisch, Band 2037, 489 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 1136 g

Reihe: Methods in Molecular Biology

This book provides broad coverage of nuclear magnetic resonance (NMR) spectroscopy-based methods and applications for the analysis of metabolites in a wide range of biological samples, from biofluids, cells, animal models, human, to plants and foods. The applications range from mechanistic understanding, biomarker discovery, environmental studies, and drug discovery to nutrition, while NMR methods include global, targeted, and isotope tracer-based techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, NMR-Based Metabolomics: Methods and Protocols serves as a wealth of information for beginners as well as advanced practitioners and also as stepping stones for further advancesin the field of metabolomics.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Raftery, Daniel

Gowda, G. A. Nagana

Fachgebiete

• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Chemie Analytische Chemie Magnetresonanz

Inhaltsverzeichnis

Overview of NMR Spectroscopy-Based Metabolomics: Opportunities and Challenges.- Analysis of Plasma, Serum, and Whole Blood Metabolites Using H NMR Spectroscopy.- Lipid Profiling Using H-NMR Spectroscopy.- High Resolution Magic Angle Spinning (HRMAS) NMR Methods in Metabolomics.- Practical Guidelines for C-Based NMR Metabolomics.- Analysis of Coenzymes and Antioxidants in Tissue and Blood Using 1D H NMR Spectroscopy.- UHPLC-QTOF-MS/MS-SPE-NMR: A Solution to the Metabolomics Grand Challenge of Higher-Throughput, Confident Metabolite Identifications.- Fast NMR Methods for Identification of Resonances and Metabolic Pathways.- Stable Isotope-Resolved Metabolomics by NMR.- Tracking Metabolic Rewiring of Cancer Metabolism in Humans Using Isotope-Resolved NMR.- NMR-Based Metabolomics in Cardiac Research.- Breast Cancer Metabolomics Using NMR.- NMR-Based Urinary Metabolomics Applications.- NMR-Based Metabolomics in Gallbladder Cancer Research.- Biomarker Discovery Using NMR-Based Metabolomics of Tissue.- NMR Metabolomics Protocols for Drug Discovery.- Practical Aspects of the Analysis of Low- and High-Field NMR Data from Environmental Samples.- NMR-Based Metabolomics of Food.- Plant Metabolomics Using NMR Spectroscopy.- Fast Quantitative 2D NMR for Untargeted and Targeted Metabolomics.- Targeted Metabolomics with Quantitative Dissolution Dynamic Nuclear Polarization.- Metabolic Profiling Using In Vivo High Field Flow NMR.- Tools for Enhanced NMR-Based Metabolomics Analysis.- Automated Tools for the Analysis of 1D and 2D NMR Spectra.- Processing and Analysis of Untargeted Multi-Cohort NMR Data.- Advanced Statistical Methods for NMR-Based Metabolomics.