Prinz Numerical Methods for the Life Scientist

Binding and Enzyme Kinetics Calculated with GNU Octave and MATLAB
1. Auflage 2011
ISBN: 978-3-642-20820-1
Verlag: Springer
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 149 Seiten, eBook

Enzyme kinetics, binding kinetics and pharmacological dose-response curves are currently analyzed by a few standard methods. Some of these, like Michaelis-Menten enzyme kinetics, use plausible approximations, others, like Hill equations for dose-response curves, are outdated. Calculating realistic reaction schemes requires numerical mathematical routines which usually are not covered in the curricula of life science. This textbook will give a step-by-step introduction to numerical solutions of non-linear and differential equations. It will be accompanied with a set of programs to calculate any reaction scheme on any personal computer. Typical examples from analytical biochemistry and pharmacology can be used as versatile templates. When a reaction scheme is applied for data fitting, the resulting parameters may not be unique. Correlation of parameters will be discussed and simplification strategies will be offered.

Zielgruppe

Research

Autoren/Hrsg.

Prinz, Heino

Inhaltsverzeichnis

Preparing the computer.- Binding equilibria.- Binding kinetics.- Least squares fit to experimental data.- Multi-parameter fits.- Comparing the two different approaches.