Primrose / Twyman Principles of Gene Manipulation and Genomics

7. Auflage 2013
ISBN: 978-1-118-65388-3
Verlag: John Wiley & Sons
Format: EPUB
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 672 Seiten, E-Book

The increasing integration between gene manipulation and genomicsis embraced in this new book, Principles of Gene Manipulationand Genomics, which brings together for the first time thesubjects covered by the best-selling books Principles of GeneManipulation and Principles of Genome Analysis &Genomics.
* Comprehensively revised, updated and rewritten to encompasswithin one volume, basic and advanced gene manipulation techniques,genome analysis, genomics, transcriptomics, proteomics andmetabolomics
* Includes two new chapters on the applications of genomics
* An accompanying website - www.blackwellpublishing.com/primrose- provides instructional materials for both student and lectureruse, including multiple choice questions, related websites, and allthe artwork in a downloadable format.
* An essential reference for upper level undergraduate andgraduate students of genetics, genomics, molecular biology andrecombinant DNA technology.

Autoren/Hrsg.

Primrose, Sandy B.

Twyman, Richard

Inhaltsverzeichnis

Preface.
1. Gene Manipulation in the Post-Genomics Era.
Part I: Fundamental Techniques of Gene Manipulation:.
2. Basic Techniques.
3. Cutting and Joining DNA Molecules.
4. Basic Biology of Plasmid and Phage Vectors.
5. Cosmids, Phasmids and Other Advanced Vectors.
6. Gene Cloning Strategies.
7. Sequencing Genes and Short Stretches of DNA.
8. Changing Genes: Site-directed Mutagenesis and ProteinEngineering.
9. Bioinformatics.
Part II: Manipulating DNA in Microbes, Plants andAnimals:.
10. Cloning in Bacteria Other Than Escherichia coli.
11. Cloning in Saccharomyces cerevisiaea and OtherFungi.
12. Gene Transfer to Animal Cells.
13. Genetic Manipulation of Animals.
14. Gene Transfer to Plants.
15. Advanced Transgenic Technology.
Part III: Genome Analysis, Genomics and Beyond:.
16. The Organization and Structure of Genomes.
17. Mapping and Sequencing Genomes.
18. Comparative Genomics.
19. Large-Scale Mutagenesis and Interference.
20. Analysis of the Transcriptome.
21. Proteomics I - Expression Analysis andCharacterization of Proteins.
22. Proteomics II - Analysis of Protein Structures.
23. Proteomics III - Protein Interactions.
24. Metabolomics and Global Biochemical Networks.
Part IV: Applications of Gene Manipulation andGenomics:.
25. Applications of Genomics: Understanding the Basis ofPolygenic Disorders and Identifying Quantitative Trait Loci.
26. Applications of Recombinant DNA Technology.
Index

Über Autor(innen)

Sandy Primrose was an academic for 10 years and a seniormanager in the biotechnology industry for 20 years. He now managesa consultancy practice focused on technology management.
Richard Twyman worked as a research scientist at theUniversity of Warwick and at the MRC Laboratory of MolecularBiology in Cambridge before taking up scientific writing as a fulltime occupation in 1998. He is currently a Visiting Fellow at theDepartment of Biology, University of York, and a freelancescientific writer and teacher.