Pound Immunochemical Protocols

2. Auflage 1998
ISBN: 978-1-59259-257-9
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 508 Seiten

Reihe: Springer Protocols

Zielgruppe

Research

Autoren/Hrsg.

Pound, John

Inhaltsverzeichnis

Production of Polyclonal Antisera.- Raising Polyclonal Antibodies Using Nitrocellulose-Bound Antigen.- Production and Characterization of Antibodies Against Synthetic Peptides.- Preparation and Testing of Monoclonal Antibodies to Recombinant Proteins.- A Simple Hollow-Fiber Bioreactor for the “In-House” Production of Monoclonal Antibodies.- Screening of Monoclonal Antibodies Using Antigens Labeled with Acetylcholinesterase.- Synthesis of Peptides for Use as Immunogens.- Use of Proteins Blotted to Polyvinylidene Difluoride Membranes as Immunogens.- Purification of Glycoproteins and Their Use as Immunogens.- IgG Purification.- Purification of Monoclonal Antibodies.- Production of Bispecif ic and Trispecific F(ab)2 and F(ab)3 Antibody Derivatives.- Preparation of Cytotoxic Antibody—Toxin Conjugates.- Immunoaffinity Purification and Quantification of Antibody—Toxin Conjugates.- Competitive ELISA.- Epitope Mapping.- Coupling of Antibodies with Biotin.- Preparation of Avidin Conjugates.- Enhanced Chemiluminescence Immunoassay.- Nonradioactive Methods for Visualization of Protein Blots.- Colloidal Gold Staining and Immunoprobing on the Same Western Blot.- Erasure of Western Blots After Autoradiographic or Chemiluminescent Detection.- Colloidal Gold Staining and Immunodetection in 2D Protein Mapping.- Enzyme-Antienzyme Method for Immunohistochemistry.- Immunohistochemical Detection of Bromodeoxyuridine-Labeled Nuclei for In Vivo Cell Kinetic Studies.- Cell Kinetic Studies Using a Monoclonal Antibody to Bromodeoxyuridine.- Immunohistochemical Detection of Cells in the Division Cycle Using Antibodies to Proliferating Cell Nuclear Antigen (PCNA).- Double Label Immunohistochemistry on Tissue Sections Using Alkaline Phosphatase and Peroxidase Conjugates.- Preparation ofColloidal Gold Probes.- Immunogold Probes in Light Microscopy.- Immunogold Probes in Electron Microscopy.- Electron Microscopic-Silver Enhancement for Double Labeling with Antibodies Raised in the Same Species.- Flow Cytometric Analysis of Cell Surface Antigen Density.- Multiple Immunofluorescence Analysis of Cells Using Flow Cytometry.- Flow Cytometric Methods of Analyzing Apoptotic Cells.- Flow Cytometric Detection of Proliferation-Associated Antigens, PCNA and Ki-67.- Sorting of Human Peripheral Blood T-Cell Subsets Using Immunomagnetic Beads.- Preparation and Use of Nonradioactive Hybridization Probes.- Cellular Human and Viral DNA Detection by Nonisotopic In Situ Hybridization.- Biotinylated Probes in Colony Hybridization.- Nonisotopic In Situ Hybridization.- Expression of Recombinant Antibody Fusion Proteins in E. coli.- Expression of Antibody Fusion Proteins in Mammalian Cells.- Antibody Screening of Bacteriophage ? gt-11 DNA Expression Libraries.- Display of Antibody Chains on Filamentous Bacteriophage.- Production of Human Fab Antibody Fragments from Phage Display Libraries.- Antibodies from Phage Display Libraries as Immunochemical Reagents.