Phillips / Shephard Cytochrome P450 Protocols

Erscheinungsjahr 2008
ISBN: 978-1-59259-580-8
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 107, 482 Seiten, eBook

Reihe: Methods in Molecular Biology

Zielgruppe

Research

Autoren/Hrsg.

Phillips, Ian R.

Shephard, Elizabeth A.

Inhaltsverzeichnis

The Role of Methods in the Discovery of the Cytochromes P450.- Cytochrome P450 Nomenclature.- Spectral Analyses of Cytochromes P450.- Purification of Cytochromes P450.- Isolation and Purification of Constitutive Forms of Microsomal Cytochrome P450.- Purification of Extrahepatic Cytochromes P450.- Purification of Cytochromes P450: Products of Bacterial Recombinant Expression Systems.- Cytochrome P450 Reconstitution Systems.- Catalytic Assays for Human Cytochrome P450.- Enzymatic Analysis of cDNA-Expressed Human CYP1A1, CYP1A2, and CYP1B1 with 7-Ethoxyresorufin as Substrate.- Spectrofluorometric Analysis of CYP2A6-Catalyzed Coumarin 7-Hydroxylation.- Determination of the CYP2B6 Component of 7-Ethoxy-4-Trifluoromethylcoumarin O-Deethylation Activity in Human Liver Microsomes.- High-Performance Liquid Chromatographic Analysis of CYP2CSCatalyzed Paclitaxel 6?-Hydroxylation.- Determination of CYP2C9-Catalyzed Diclofenac 4?-Hydroxylation by High-Performance Liquid Chromatography.- CYP2Cl g-Mediated (S)-Mephenytoin 4’-Hydroxylation Assayed by High-Performance Liquid Chromatography with Radiometric Detection.- CYP2D6-Dependent Bufuralol 1’-Hydroxylation Assayed by Reversed-Phase lon-Pair High-Performance Liquid Chromatography with Fluorescence Detection.- Spectrophotometric Analysis of Human CYP2E1-Catalyzed p-Nitrophenol Hydroxylation.- Thin-Layer Chromatographic Analysis of Human CYP3A-Catalyzed Testosterone 6?-Hydroxylation.- Determination of CYP4A11-Catalyzed Lauric Acid 12-Hydroxylation by High-Performance Liquid Chromatography with Radiometric Detection.- An Isocratic High-Performance Liquid Chromatographic Assay for CYP7Al-Catalyzed Cholesterol 7?-Hyciroxylation.- Use of 7-Ethoxycoumarinto Monitor Multiple Enzymesin the Human CYP1, CYP2, and CYP3 Families.-Expression of Eukaryotic Cytochromes P450 in E. coli.- Expression of Mammalian Cytochromes P450 in Yeast.- Expression of Cytochromes P450 in a Baculovirus System.- Expression of Cytochromes P450 in Mammalian Cells.- Monoclonal Antibodies to Cytochromes P450.- Targeting Antipeptide Antibodies Towards Cytochrome P450 Enzymes.- Signals for Retention of Cytochrome P450 in the Endoplasmic Reticulum.- Quantification of Cytochrome P450 Gene Expression by RNase Protection Analysis.- Hepatocyte Cultures in Drug Metabolism and Toxicological Research and Testing.- Isolation of Rat Hepatocytes.- Collagen-Gel Cultures of Rat Hepatocytes:.- Rat Hepatocyte Cultures.- Isolation of Periportal and Pericentral Hepatocytes.- Perifusion Culture of Hepatocytes.- Human Hepatocyte Culture.- Reconstruction of Human Epidermis In Vitro.- Gene Transfer and Expression.- Lipid-Mediated Gene Transfer into Normal Adult Human Hepatocytes in Primary Culture.- Identification of Regulatory Protein- Binding Sites in Cytochrome P450 Genes by Means of the Gel- Retardation Assay.- Cytochrome P450 Gene Regulation.- Analysis of Cytochrome P450 Polymorphisms.- Induction of Cytochrome P4501A (CYP1A) in Fish A Biomarker for Environmental Pollution.- Generation of Allelic Series in Mice by Gene Targeting.