Peterson / Loring / Wesselschmidt Human Stem Cell Manual

A Laboratory Guide
1. Auflage 2011
ISBN: 978-0-08-054988-0
Verlag: Elsevier Science & Techn.
Format: EPUB
Kopierschutz: 6 - ePub Watermark

E-Book, Englisch, 488 Seiten

Stem cells are self-replicating and undifferentiated, meaning their function is not yet cell, tissue, or organ-specific. Due to the unique nature of these cells, research into their biology and function holds great promise for therapeutic applications through replacement or repair of diseased and damaged cells. This reader-friendly manual provides a practical 'hands on' guide to the culture of human embryonic and somatic stem cells. By presenting methods for embryonic and adult lines side-by-side, the authors lay out an elegant and unique path to understanding the science of stem cell practice. The authors begin with a broad-based introduction to the field, and also review legal and regulatory issues and patents. Each experimental strategy is presented with an historical introduction, detailed method, discussion of alternative methods, and common pitfalls. This lab guide for researchers also serves as a textbook for undergraduate and graduate students in laboratory courses.

Autoren/Hrsg.

Peterson, Suzanne

Loring, Jeanne F.

Wesselschmidt, Robin L.

Schwartz, Philip H.

Inhaltsverzeichnis

1;Cover;1
2;TOC$Contents;6
3;Foreword by James Battey;10
4;Foreword by Jeannie Fontana;12
5;Preface by Jeanne Loring;14
6;Preface by Robin L. Wesselschmidt;16
7;Preface by Philip H. Schwartz;18
8;List of Contributors;20
9;Part I: Basic Methods in Stem Cell Culture;26
9.1;CH$Chapter 1 Human Embryonic Stem Cell Culture;28
9.2;CH$Chapter 2 Human Feeder Cells, Feeder-free, and Defined Culture Systems;43
9.3;CH$Chapter 3 Mouse Embryonic Fibroblast Feeder Cells;59
9.4;CH$Chapter 4 Cryopreservation of Human Embryonic Stem Cells;72
10;Part II: Characterization of Stem Cells;82
10.1;CH$Chapter 5 Classical Cytogenetics: Karyotyping;84
10.2;CH$Chapter 6 Spectral Karyotyping and Fluorescent in situ Hybridization;96
10.3;CH$Chapter 7 Genotype and Epigenotype by Single Nucleotide Polymorphism (SNP) Analysis;110
10.4;CH$Chapter 8 Flow Cytometric Analysis of Human Embryonic Stem Cells;121
10.5;CH$Chapter 9 Immunocytochemical Analysis of Stem Cells;133
10.6;CH$Chapter 10 Characterization of Stem Cells Using Reverse Transcriptase Polymerase Chain Reaction;152
10.7;CH$Chapter 11 Gene Expression Profiling of Stem Cells by Microarray;174
10.8;CH$Chapter 12 Generation of Human Embryonic Stem Cell-derived Teratomas;187
10.9;CH$Chapter 13 Characterization of Human Embryonic Stem Cell-derived Teratomas;196
11;Part III: Differentiation of Human Embryonic Cells;208
11.1;CH$Chapter 14 Embryoid Bodies and Neuroepithelial Development;210
11.2;CH$Chapter 15 Motor Neuron and Dopamine Neuron Differentiation;224
11.3;CH$Chapter 16 Oligodendrocyte Differentiation from Human Embryonic Stem Cells;235
11.4;CH$Chapter 17 Cardiac Development of Human Embryonic Stem Cells;252
11.5;CH$Chapter 18 Hematopoiesis from Human Embryonic Stem Cells;263
12;Part IV: Genetic Manipulation of Stem Cells;278
12.1;CH$Chapter 19 Genetic Manipulation of Human Embryonic Stem Cells: Lentivirus Vectors;280
12.2;CH$Chapter 20 Genetic Manipulation of Embryonic Stem Cells;292
13;Part V: Advanced Methods;314
13.1;CH$Chapter 21 Derivation of Embryonic Stem Cells from Human Blastocysts;316
13.2;CH$Chapter 22 Neural Progenitor Cell Culture;334
13.3;CH$Chapter 23 Stem Cell Transplantation in the Brain;357
13.4;CH$Chapter 24 In Vitro Fertilization;376
13.5;CH$Chapter 25 Functional Characterization of Neurons;398
14;Part VI: Setting up a Laboratory;412
14.1;CH$Chapter 26 Setting up a Facility for Human Embryonic Stem Cell Research;414
15;Part VII: Stem Cells and Society;440
15.1;CH$Chapter 27 Intellectual Property: Owning the Stem Cell;442
15.2;CH$Chapter 28 Ethical Concerns for Stem Cell Research;451
15.3;CH$Chapter 29 Guidelines for Embryonic Stem Cell Research Oversight (ESCRO) Committees;462
16;Abbreviations;474
17;IDX$Index;478
17.1;A;478
17.2;B;478
17.3;C;478
17.4;D;479
17.5;E;479
17.6;F;480
17.7;G;480
17.8;H;481
17.9;I;481
17.10;K;482
17.11;L;482
17.12;M;482
17.13;N;483
17.14;O;483
17.15;P;483
17.16;Q;483
17.17;R;484
17.18;S;484
17.19;T;485
17.20;U;486
17.21;V;486
17.22;W;486
17.23;X;486
17.24;Y;486