Packer Superoxide Dismutase

1. Auflage 2002
ISBN: 978-0-08-049697-9
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band Volume 349, 400 Seiten, Web PDF

Reihe: Methods in Enzymology

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant todaytruly an essential publication for researchers in all fields of life sciences.

Autoren/Hrsg.

Packer, Lester

Inhaltsverzeichnis

1;Cover;1
2;Table of Contents;6
3;Contributors to Volume 349;10
4;Preface;16
5;Volume in Series;18
6;Section I: Superoxide Reactions and Mechanisms;38
6.1;Chapter 1. Quantitation of Intracellular Free Iron by Electron Paramagnetic Resonance Spectroscopy;40
6.2;Chapter 2. Aconitase: Sensitive Target and Measure of Superoxide;46
6.3;Chapter 3. Reactions of Manganese Porphyrins and Manganese-Superoxide Dismutase with Peroxynitrite;60
6.4;Chapter 4. Superoxide Dismutase Kinetics;75
6.5;Chapter 5. Analysis of Cu, ZnSOD Conformational Stability by Differential Scanning Calorimetry;86
6.6;Chapter 6. Catalytic Pathway of Manganese Superoxide Dismutase by Direct Observation of Superoxide;98
6.7;Chapter 7. Extracellular Superoxide Dismutase;111
6.8;Chapter 8. Prokaryotic Manganese Superoxide Dismutases;117
6.9;Chapter 9. Nickel-Containing Superoxide Dismutase;127
6.10;Chapter 10. Reversible Conversion of Nitroxyl Anion to Nitric Oxide;138
6.11;Chapter 11. Purification and Determination of Activity of Mitochondrial Cyanide-Sensitive Superoxide Dismutase in Rat Tissue Extract;143
6.12;Chapter 12. Studies of Metal-Binding Properties of Cu,Zn Superoxide Dismutase by Isothermal Titration Calorimetry;152
6.13;Chapter 13. Superoxide Reductase from Desulfoarculus baarsii;160
6.14;Chapter 14. Enzyme-Linked Immunosorbent Assay for Human and Rat Manganese Superoxide Dismutases;166
7;Section II: Mutants, Knockouts, Transgenics;180
7.1;Chapter 15. Investigating Phenotypes Resulting from a Lack of Superoxide Dismutase in Bacterial Null Mutants;182
7.2;Chapter 16. Bacterial Superoxide Dismutase and Virulence;192
7.3;Chapter 17. Superoxide Dismutase Null Mutants of Baker’s Yeast, Saccharomyces cerevisiae;204
7.4;Chapter 18. Measurement of FreeŽ or Electron Paramagnetic Resonance-Detectable Iron in Whole Yeast Cells as Indicator of Superoxide Stress;210
7.5;Chapter 19. Transgenic Superoxide Dismutase Overproducer: Murine;217
7.6;Chapter 20. Transgenic and Mutant Mice for Oxygen Free Radical Studies;228
7.7;Chapter 21. Overexpression of Cu,ZnSOD and MnSOD in Transgenic Drosophila;250
8;Section III: Superoxide Dismutase Mimics;258
8.1;Chapter 22. Manganese Porphyrins and Related Compounds as Mimics of Superoxide Dismutase;260
8.2;Chapter 23. Superoxide Dismutase Mimics: Antioxidative and Adverse Effects;271
8.3;Chapter 24. Superoxide Reductase Activities of Neelaredoxin and Desulfoferrodoxin Metalloproteins;280
8.4;Chapter 25. Purification and Preparation of Prion Protein: Synaptic Superoxide Dismutase;295
9;Section IV: In Vivo Sources, Cell Signaling;306
9.1;Chapter 26. Mitochondrial Superoxide Anion Production and Release into Intermembrane Space;308
9.2;Chapter 27. Measurement of Superoxide Radical and Hydrogen Peroxide Production in Isolated Cells and Subcellular Organelles;317
9.3;Chapter 28. Biochemical Assay of Superoxide Dismutase Activity in Drosophila;324
9.4;Chapter 29. Transcriptional Regulation and Environmental Induction of Gene Encoding Copper- and Zinc- Containing Superoxide Dismutase;330
9.5;Chapter 30. Transcription Regulation of Human Manganese Superoxide Dismutase Gene;343
9.6;Chapter 31. Assessment of Oxidants in Mitogen-Activated Protein Kinase Activation;350
9.7;Chapter 32. Assaying Binding Capacity of Cu,ZnSOD and MnSOD: Demonstration of Their Localization in Cells and Tissues;358
10;Section V: Superoxide Dismutase in Aging and Disease Therapy;366
10.1;Chapter 33. Superoxide Dismutase in Aging and Disease: An Overview;368
10.2;Chapter 34. Tissue-Specific Mitochondrial Production of H2O2: Its Dependence on Substrates and Sensitivity to Inhibitors;378
10.3;Chapter 35. Targeting Superoxide Dismutase to Critical Sites of Action;383
10.4;Chapter 36. InVitro Quantitation of Biological Superoxide and Hydrogen Peroxide Generation;391
11;Author Index;400
12;Subject Index;428