Oncogene-Induced Senescence

Methods and Protocols
1. Auflage 2017
ISBN: 978-1-4939-6668-4
Verlag: Springer US

Buch, Englisch, Band 1534, 220 Seiten, HC runder Rücken kaschiert, Format (B × H): 183 mm x 260 mm, Gewicht: 5855 g

Reihe: Methods in Molecular Biology

This book offers in a single volume a unique collection of the state-of the-art experimental procedures utilized for the induction, detection, and modeling of this complex cellular program of oncogene-induced senescence. The book encompasses protocols for studying this multi-step program in human specimens and a variety of experimental models including cultured mammalian cells, laboratory mice, and Drosophila melanogaster, as well as offering a description of high throughput approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Oncogene-Induced Senescence: Methods and Protocols represents a valuable asset for a wide audience of medical oncologists and researchers in the fields of oncology, molecular and cellular biology, biochemistry, and animal development.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Nikiforov, Mikhail A.

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Onkologie, Krebsforschung

Inhaltsverzeichnis

The Immortal Senescence.- Senescence Phenotypes Induced by Ras in Primary Cells.- Cellular Model of p21-Induced Senescence.- Detecting Markers of Therapy-Induced Senescence in Cancer Cells.- Genome-Wide miRNA Screening for Genes Bypassing Oncogene-Induced Senescence.- Detection of Dysfunctional Telomeres in Oncogene-Induced Senescence.- RT-qPCR Detection of Senescence-Associated Circular RNAs.- Autophagy Detection During Oncogene-Induced Senescence Using Fluorescence Microscopy.- Detecting the Senescence-Associated Secretory Phenotype (SASP) by High Content Microscopy Analysis.- Sudan Black B, the Specific Histochemical Stain for Lipofuscin: A Novel Method to Detect Senescent Cells.- Using [U-C]-Glucose Tracer to Study Metabolic Changes in Oncogene-Induced Senescence Fibroblasts.- Detection of the Ubiquitinome in Cells Undergoing Oncogene-Induced Senescence.- Detection of Reactive Oxygen Species inCells Undergoing Oncogene-Induced Senescence.- Detection of Senescent Cells by Extracellular Markers Using a Flow Cytometry-Based Approach.- Metabolic Changes Investigated by Proton NMR Spectroscopy in Cells Undergoing Oncogene-Induced Senescence.- Detection of Nucleotide Disbalance in Cells Undergoing Oncogene-Induced Senescence.- Senescence-Like Phenotypes in Human Nevi.- Detection of Oncogene-Induced Senescence In Vivo.- Detection of Senescence Markers During Mammalian Embryonic Development.- Induction and Detection of Oncogene-Induced Cellular Senescence in Drosophila.