Buch, Englisch, 544 Seiten, Format (B × H): 172 mm x 248 mm, Gewicht: 1186 g
From Bioanalysis to Biomedicine
Buch, Englisch, 544 Seiten, Format (B × H): 172 mm x 248 mm, Gewicht: 1186 g
ISBN: 978-3-527-35173-2
Verlag: Wiley-VCH GmbH
DNA Nanotechnology for Cell Research
Comprehensive coverage of DNA nanotechnology with a focus on its biomedical applications in disease diagnosis, gene therapy, and drug delivery
Bringing together multidisciplinary aspects of chemical, material, and biological engineering, DNA Nanotechnology for Cell Research: From Bioanalysis to Biomedicine presents an overview of DNA nanotechnology with emphasis on a variety of different applications in cell research and engineering, covering a unique collection of DNA nanotechnology for fundamental research and engineering of living cells, mostly in cellulo and in vivo, for the first time. Broad coverage of this book ranges from pioneering concepts of DNA nanotechnology to cutting-edge reports regarding the use of DNA nanotechnology for fundamental cell science and related biomedical engineering applications in sensing, bioimaging, cell manipulation, gene therapy, and drug delivery.
The text is divided into four parts. Part I surveys the progress of functional DNA nanotechnology tools for cellular recognition. Part II illustrates the use of DNA-based biochemical sensors to monitor and image intracellular molecules and processes. Part III examines the use of DNA to regulate biological functions of individual cells. Part IV elucidates the use of DNA nanotechnology for cell-targeted medical applications.
Sample topics covered in DNA Nanotechnology for Cell Research include: - Selections and applications of functional nucleic acid toolkits, including DNA/RNA aptamers, DNAzymes, and riboswitches, for cellular recognition, metabolite detection, and liquid biopsy.
- Developing intelligent DNA nanodevices implemented in living cells for amplified cell imaging, smart intracellular sensing, and in cellulo programmable biocomputing.
- Harnessing dynamic DNA nanotechnology for non-genetic cell membrane engineering, receptor signaling reprogramming, and cellular behavior regulation.
- Construction of biocompatible nucleic acid nanostructures as precisely controlled vehicles for drug delivery, immunotherapy, and tissue engineering.
Providing an up-to-date tutorial style overview along with a highly valuable in-depth perspective, DNA Nanotechnology for Cell Research is an essential resource for the entire DNA-based nanotechnology community, including analytical chemists, biochemists, materials scientists, and bioengineers.
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Weitere Infos & Material
Preface
PART I. DNA Nanotechnology for Cellular Recognition (Cell SELEX, cell surface engineering)
1. DEVELOPING DNA APTAMER TOOLBOX FOR CELL RESEARCH
1.1 Cells and their complexity
1.2 DNA aptamers' characteristics and advantages
1.3 On-demand synthesis and screening of DNA aptamers
1.4 Toward a toolbox of DNA aptamers for cellular applications
1.5 Summary and Outlook
2. BACTERIAL DETECTION WITH FUNCTIONAL NUCLEIC ACIDS: ESCHERICHIA COLI AS A CASE STUDY
2.1 General Introduction on Bacteria
2.2 E. coli
2.3 Conventional Methods for General E. coli Detection
2.4 Biosensors for E. coli Detection
2.5 Conclusion
3. FROM LIGAND-BINDING APTAMERS TO MOLECULAR SWITCHES
3.1 Aptamers can be generated by SELEX
3.2 Various subtypes of SELEX have been invented
3.3 Riboswitches are natural RNA aptamers carrying expression platforms
3.4 Riboswitches use various mechanisms to regulate gene expression
3.5 Riboswitches are potential drug targets
3.6 Fusing aptamer with expression platform to construct artificial RNA switches
3.7 Conclusions
4. DNA NANOTECHNOLOGY-BASED MICROFLUIDICS FOR LIQUID BIOPSY
4.1 Introduction
4.2 DNA nanotechnology-based microfluidics for isolation of circulating targets
4.3 DNA nanotechnology-based microfluidics for release and detection of circulating targets
4.4 DNA-assisted microfluidics for single cell/vesicle analysis
4.5 Summary and Outlook
5. SPATIOTEMPORAL CONTROLLED CELL MEMBRANE ENGINEERING USING DNA NANOTECHNOLOGY
5.1 Background
5.2 DNA Modifications on the External Cell Membrane Surface
5.4 Perspectives
PART II. DNA Nanotechnology for Cell Imaging and Intracellular Sensing
6. METAL-DEPENDENT DNAZYMES FOR CELL SURFACE ENGINEERING AND INTRACELLULAR BIOIMAGING
6.1 Cellular Surface Engineering and Intracellular Bioimaging Show Great Potential in Biological and Medical Research
6.2 Metal-Specific DNAzymes: a Suitable Choice for Artificial Manipulation of Living Cells
6.3 Cell Surface Engineering by Programmable DNAzymes
6.4 Intracellular Imaging of Metal Ions with DNAzyme-Based Biosensors
6.5 Conclusion
7. DNA NANOMOTORS FOR BIOIMAGING IN LIVING CELLS
8. ILLUMINATING RNA IN LIVE CELLS WITH INORGANIC NANOPARTICLES-BASED DNA SENSOR TECHNOLOGY
8.1 RNA Detection and Imaging
8.2 RNA Imaging Based on Direct Hybridization
8.3 RNA Imaging Based on Strand Displacement Reactions
8.4 Signal-amplified RNA Imaging
8.5 Spatiotemporally Controlled RNA Imaging in Live Cells
8.6 Conclusion
9. BUILDING DNA COMPUTING SYSTEM FOR SMART BIOSENSING AND CLINICAL DIAGNOSIS
9.1 DNA computing
9.2 DNA-based computing devices for biosensing
9.3 DNA computing for clinical diagnosis
9.4 Conclusion
10. INTELLIGENT SENSE-ON-DEMAND DNA CIRCUITS FOR AMPLIFIED BIOIMAGING IN LIVING CELLS
10.1 DNA Circuit: The Promising Technique for Bioimaging
10.2 Non-enzymatic DNA Circuits
10.3 Intelligent Integrated DNA Circuits for Amplified Bioimaging
10.4 Stimuli-responsive DNA Circuits for Reliable Bioimaging
10.5 Conclusion and Perspectives
11. DNA NANOSCAFFOLDS FOR BIOMACROMOLECULES ORGANIZATION AND BIOIMAGING APPLICATIONS
11.1 Introduction
11.2 Assembly of DNA-scaffolded biomacromolecules
11.3 Application of DNA nanoscaffold for regulation of enzyme cascade reaction
11.4 DNA nanostructures empowered bioimaging technologies
11.5 Summary and outlook
PART III. DNA Nanotechnology for Regulation of Cellular Functions
12. ADOPTING NUCLEIC ACID NANOTECHNOLOGY FOR GENETIC REGULATION IN VIVO
12.1 Introduction
12.2 Toehold-mediated strand displacement: switching nucleic acids with nucleic acids
12.3 Toehold riboregulators and related systems
12.4 Applying nucleic acid nanotechnology to CRISPR and RNA interference
12.5 Delivery of nucleic acid devices, in vivo production, and challenges for in vivo operation
12.6 Conclusion & Outlook
13. CELL MEMBRANE FUNCTIONALIZATION VIA NUCLEIC ACID TOOLS FOR VISUALIZATION AND REGULATION OF CELLULAR RECEPTORS
13.1 Nucleic Acid-based Functionalization Strategies: From Receptor Information to DNA Probes
13.2 Uncovering Molecular Information of Cellular Receptors
13.3 Governing Cellular Receptors-Mediated Signal Transduction
13.4 Conclusion
14. HARNESSING DNA NANOTECHNOLOGY FOR NON-GENETIC MANIPULATION AND FUNCTIONALIZATION OF CELL SURFACE RECEPTOR
14.1 Introduction
14.2 Principle of DNA-enabled molecular engineering for receptor regulation
14.3 DNA nanodevices for programming receptor function
14.4 Elaborate and intelligent DNA nanodevices reprogramming receptor function
14.5 Conclusions and perspectives
15. DNA-BASED CELL SURFACE ENGINEERING FOR PROGRAMMING MULTIPLE CELL-CELL INTERACTIONS
15.1 DNA Nanotechnology: The Tool of Choice for Programming Cell-Cell Interactions
15.2 Modifying Cell Surface with DNA
15.3 Programming Cell-Cell Interactions by DNA Nanotechnology
15.4 Conclusion
16. DESIGNER DNA NANOSTRUCTURES AND THEIR CELLULAR UPTAKE BEHAVIORS
16.1 Introduction
16.2 DNA Nanotechnology
16.3 Pathways of Cell Endocytosis
16.4 Analysis of DNA Nanostructures' Cellular Uptake behaviors
PART IV. DNA Nanotechnology for Cell-Targeted Medical Applications
17. TOWARDS PRODUCTION OF NUCLEIC ACID NANOSTRUCTURES IN LIFE CELLS AND THEIR BIOMEDICAL APPLICATIONS
17.1 DNA Nanostructures
17.2 RNA Nanostructures
17.3 Applications
17.4 Conclusion
18. ENGINEERING NUCLEIC ACID STRUCTURES FOR PROGRAMMABLE INTRACELLULAR BIOCOMPUTATION
19. DNA SUPRAMOLECULAR HYDROGELS FOR BIOMEDICAL APPLICATIONS
19.1 Introduction
19.2 Classification and Preparation of DNA supramolecular hydrogels
19.3 Biomedical application of DNA supramolecular hydrogels
19.4 Conclusions and perspectives
20. ROLLING CIRCLE AMPLIFICATION-BASED DNA-NANOTECHNOLOGY FOR CELL RESEARCH
20.1 Introduction
20.2 Principle and synthetic methods of RCA
20.3 RCA-based DNA nanotechnology for cell separation
20.4 RCA-based DNA nanotechnology for nucleic acid drug delivery
20.5 Conclusion
21. PRECISE INTEGRATION OF THERAPEUTICS IN DNA-BASED NANOMATERIAL FOR CANCER TREATMENTS
21.1 DNA-based nanomaterials in biomedicines
21.2 Strategies on constructing DNA-based DDSs
21.3 Precise integration of therapeutics into DNA-based DDSs to achieve synergistic cancer treatment
