Microarray Technology and Its Applications

2005
ISBN: 978-3-540-22931-5
Verlag: Springer Berlin Heidelberg

Buch, Englisch, 380 Seiten, Format (B × H): 160 mm x 241 mm, Gewicht: 844 g

Reihe: Biological and Medical Physics, Biomedical Engineering

Ithasbeenstatedthatourknowledgedoublesevery20years,butthatmaybe an understatement when considering the Life Sciences. A series of discoveries and inventions have propelled our knowledge from the recognition that DNA isthegeneticmaterialtoabasicmolecularunderstandingofourselvesandthe living world around us in less than 50 years. Crucial to this rapid progress was thediscoveryofthedouble-helicalstructureofDNA,whichlaidthefoundation forallhybridizationbasedtechnologies. Thediscoveriesofrestrictionenzymes, ligases, polymerases, combined with key innovations in DNA synthesis and sequencing ushered in the era of biotechnologyas a new science with profound sociological and economic implications that are likely to have a dominating in?uence on the development of our society during this century. Given the process by which science builds on prior knowledge, it is perhaps unfair to single out a few inventions and credit them with having contributed most to thisavalancheofknowledge. Yet,therearesurelysomethatwillberecognized as having had a more profound impact than others, not just in the furthering of our scienti?c knowledge, but by leveraging commercial applications that provide a tangible return to our society. The now famous Polymerase Chain Reaction, or PCR, is surely one of those, as it has uniquely catalyzed molecular biology during the past 20 years, and continues to have a signi?cant impact on all areas that involve nucleic acids, ranging from molecular pathology to forensics. Ten years ago micro- ray technology emerged as a new and powerful tool to study nucleic acid - quences in a highly multiplexed manner, and has since found equally exciting and useful applications in the study of proteins, metabolites, toxins, viruses, whole cells and even tissues.

Zielgruppe

Research

Autoren/Hrsg.

Nicolau, Dan V.

Müller, Uwe R.

Fachgebiete

• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie Industrielle Biotechnologie
• Technische Wissenschaften Sonstige Technologien | Angewandte Technik Medizintechnik, Biomedizintechnik
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Medizintechnik, Biomedizintechnik, Medizinische Werkstoffe
• Naturwissenschaften Physik Physik Allgemein Theoretische Physik, Mathematische Physik, Computerphysik
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Physik Physik Allgemein Geschichte der Physik
• Naturwissenschaften Physik Physik Allgemein Experimentalphysik
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Humangenetik

Inhaltsverzeichnis

General Microarray Technologies.- Array Formats.- Biomolecules and Cells on Surfaces — Fundamental Concepts.- Surfaces and Substrates.- Reagent Jetting Based Deposition Technologies for Array Construction.- Manufacturing of 2-D Arrays by Pin-printing Technologies.- Nanoarrays.- The Use of Microfluidic Techniques in Microarray Applications.- Labels and Detection Methods.- Marker-free Detection on Microarrays.- DNA Microarrays.- Analysis of DNA Sequence Variation in the Microarray Format.- High Sensitivity Expression Profiling.- Applications of Matrix-CGH (Array-CGH) for Genomic Research and Clinical Diagnostics.- Analysis of Gene Regulatory Circuits.- Protein Microarrays.- Protein, Antibody and Small Molecule Microarrays.- Photoaptamer Arrays for Proteomics Applications.- Biological Membrane Microarrays.- Cell & Tissue Microarrays.- Use of Reporter Systems for Reverse Transfection Cell Arrays.- Whole Cell Microarrays.- Tissue Microarrays for Miniaturized High-Throughput Molecular Profiling of Tumors.- Application of Microarray Technologies for Translational Genomics.