E-Book, Englisch, 221 Seiten, eBook
Reihe: Springer Lab Manuals
Newman / Hobot Resin Microscopy and On-Section Immunocytochemistry
Erscheinungsjahr 2012
ISBN: 978-3-642-97481-6
Verlag: Springer
Format: PDF
Kopierschutz: 1 - PDF Watermark
E-Book, Englisch, 221 Seiten, eBook
Reihe: Springer Lab Manuals
ISBN: 978-3-642-97481-6
Verlag: Springer
Format: PDF
Kopierschutz: 1 - PDF Watermark
For the first time, fixation, resin embedding and immunocytochemistry, the subjects of innumerable publications, have been organised into a comprehensive and coherent scheme showing how the various methodologies interrelate. Commercially available resins and their mode of use are described, and the book considers the advantages for cytochemistry and immunocytochemistry of matching tissue fixation to processing and resin embedding. On-section single and double immunolabelling methods are covered, using immunocolloidal gold and immunoperoxidase.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Weitere Infos & Material
I: Resin Embedding.- 1 The Strategic Approach.- 2 The Resins.- 3 Resin Embedding Protocols for Chemically Fixed Tissue.- 4 Cryotechniques.- 5 Methods for Resin Polymerisation.- 6 Handling Resin Blocks.- II: On-Section Immunolabelling.- 7 Strategies in Immunolabelling.- 8 General Considerations.- 9 Immunolabelling Protocols for Resin Sections.- 10 Resin Embedding and Immunolabelling.- Appendix I.- Examples of Typical Resin Embedding Regimes for Immunocytochemistry.- Appendix II.- List of Suppliers.- References.