Microbial Systems Biology

Methods and Protocols
2. Auflage 2022
ISBN: 978-1-0716-1587-4
Verlag: Springer US

Buch, Englisch, Band 2349, 385 Seiten, Format (B × H): 178 mm x 254 mm, Gewicht: 750 g

Reihe: Methods in Molecular Biology

This second edition provides new and updated chapters describing the utility and attributes of different tools that are used for studying microbial systems. Chapters detail a number of methods, including in silico system level analyses by MetaFlux, Kbase genome-scale model builder, COBRA toolbox, NanoSIP, and PAMMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Microbial Systems Biology: Methods and Protocols, Second Edition aims to introduce and aid scientists in using the various tools that are currently available for analysis, modification, and utilization of microbial organisms.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Navid, Ali

Fachgebiete

• Naturwissenschaften Biowissenschaften Mikrobiologie
• Naturwissenschaften Biowissenschaften Angewandte Biologie Bioinformatik

Inhaltsverzeichnis

Parallel Accelerator and Molecular Mass Spectrometry Measurement of Carbon-14 Labeled Analytes.- Fast sampling of the cellular metabolome.- Investigation of Protein-Lipid Interactions Using Native Mass Spectrometry.- Western Blot Processing Optimization: The Perfect Blot.- FISHing on a Budget.- NanoSIP: NanoSIMS Applications for Microbial Biology.- Construction of Metatranscriptomic Libraries for 5' end Sequencing of rRNAs for Microbiome Research.- Computational Approaches for Designing Highly Specific and Efficient sgRNAs.- Complex Network Analysis in Microbial Systems: Theory and Examples.- Prokaryotic Genome Annotation.- Functional Annotation from Structural Homology.- Metabolic Modeling with MetaFlux.- Application of the Metabolic Modeling Pipeline in KBase to Categorize Reactions, Predict Essential Genes, And Predict Pathways in an Isolate Genome.- Curating COBRA Models of Microbial Metabolism.- A Beginner’s Guide to the COBRA Toolbox.- Rules of Engagement: A Guide to Developing Agent-Based Models.