E-Book, Englisch, Band 84, 231 Seiten, eBook
Reihe: Neuromethods
Nägerl / Triller Nanoscale Imaging of Synapses
2014
ISBN: 978-1-4614-9179-8
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark
New Concepts and Opportunities
E-Book, Englisch, Band 84, 231 Seiten, eBook
Reihe: Neuromethods
ISBN: 978-1-4614-9179-8
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark
Synapses underlie rapid and flexible neural communication in the brain and they hold the key to understanding higher brain functions in health and disease. Because they are very small and highly dynamic, it is very difficult to study them with traditional techniques. Fortunately, recent ground-breaking advances in optical microscopy (e.g. STED, PALM, STORM, SIM) have greatly improved our ability to image living synapses at the nanoscale, even down to the level of single molecules. The proposed volume brings together leading researchers to review these exciting new techniques and their application in neurobiological research. It will explain and discuss the basic principles behind the various superresolution modalities, how they are implemented, what their scope and limitations are etc. In addition, several key research discoveries on synapses enabled by these novel approaches will be highlighted.
Zielgruppe
Research
Autoren/Hrsg.
Weitere Infos & Material
Preface.- Nanoscale imaging of protein molecules at the postsynaptic densit.- Electron tomography for the study of synaptic ultrastructure in fixed brain sections.- Time-resolved electron microscopy: coupling optogenetics with high-pressure freezing.- Investigating AMPA receptors diffusion and nanoscale organization at synapses with high density single molecule tracking methods.- Visualising the ultra-structures and dynamics of synapses by single molecule nanoscopy.- Live-cell PALM of intracellular proteins in neurons.- Monitoring nanoscale mobility of small molecules with time-resolved fluorescence anisotropy imaging.- Imaging signalling transduction in single dendritic spines.- Imaging-based measures of synaptic tenacity.- Zooming in on the (peri)synaptic extracellular matrix.- Two-photon STED microscopy for imaging synapses and glia in acute brain slices.- Index.
