Nadeau Introduction to Experimental Biophysics, Second Edition

Biological Methods for Physical Scientists
2. Auflage 2014
ISBN: 978-1-317-97452-9
Verlag: CRC Press
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 790 Seiten

Reihe: Foundations of Biochemistry and Biophysics

This book provides a starting point for any student or researcher in the physical sciences to gain firm grounding in the techniques employed in molecular biophysics and quantitative biology. As with the first edition, the author will take a practical approach, sharing expert tips and insider’s knowledge to simplify potentially confusing techniques as much as possible. The reader will be guided through the basics of experimental set-up, followed by an easy-to-follow example carried out from start to finish, with hints for how to get things right. The emphasis is on building comfort with getting hands ‘wet’ with basic methods, and understanding how to adapt them to address other questions.

Autoren/Hrsg.

Nadeau, Jay L.

Inhaltsverzeichnis

Introduction and Background

Basic Molecular Cloning of DNA and RNA

Expression of Genes in Bacteria, Yeast, and Cultured Mammalian Cells

Advanced Topics in Molecular Biology

Protein Expression Methods

Protein Crystallization

Introduction to Biological Light Microscopy

Advanced Light Microscopy Techniques

Advanced Topics in Microscopy II: Holographic Microscopy

Quantitative Cell Culture Techniques

Semiconductor Nanoparticles (Quantum Dots)

Gold Nanoparticles

Advanced Topics in Gold Nanoparticles: Biomedical Applications

Surface Functionalization Techniques

Electrophysiology

Spectroscopy Tools and Techniques

Introduction to Nanofabrication

Common solutions

Common Media

Restriction Endonucleases

Common Enzymes

Fluorescent Dyes & Quenchers

Fluorescent Proteins

Über Autor(innen)

Jay L. Nadeau is a research professor in the Graduate Aerospace Laboratories (GALCIT) at the California Institute of Technology. Prior to Caltech, she was associate professor of biomedical engineering and physics at McGill University (2004–2015). Her research interests include nanoparticles, fluorescence imaging, and development of instrumentation for the detection of life elsewhere in the solar system.

She has published over 70 papers on topics ranging from theoretical condensed matter physics to experimental neurobiology to the development of anticancer drugs and, in the process, has used almost every technique described in this book. Her work has been featured in New Scientist, Highlights in Chemical Biology, Radio Canada’s Les Années Lumière, Le Guide des Tendances, and in educational displays in schools and museums. Her research group features chemists, microbiologists, roboticists, physicists, and physician-scientists, all learning from each other and hoping to speak each other’s language. A believer in bringing biology to physicists as well as physics to biologists, she has created two graduate-level courses: methods in molecular biology for physical scientists and mathematical cellular physiology. She has also taught pharmacology in the medical school and was one of the pioneers in the establishment of multiple mini-interviews for medical school admission.

She retains an adjunct position at McGill, and has collaborators in industry and academia in the United States, Europe, Australia, and Japan. She has given several dozen invited talks at meetings of the American Chemical Society, American Geophysical Union, the International Society for Optics and Photonics (SPIE), the Committee on Space Research, the American Association of Physics Teachers (AAPT), and many others. Before McGill, she was a member of the Jet Propulsion Laboratory’s Center for Life Detection, and previous to that a Burroughs-Wellcome postdoctoral scholar in the laboratory of Henry A. Lester at Caltech. She received her PhD in physics from the University of Minnesota in 1996.